SIN3A Polyclonal antibody
SIN3A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14638-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells |
Positive IP detected in | Jurkat cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
14638-1-AP targets SIN3A in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SIN3A fusion protein Ag6219 |
Full Name | SIN3 homolog A, transcription regulator (yeast) |
Calculated Molecular Weight | 145 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | BC066364 |
Gene Symbol | SIN3A |
Gene ID (NCBI) | 25942 |
RRID | AB_2301741 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for SIN3A antibody 14638-1-AP | Download protocol |
IF protocol for SIN3A antibody 14638-1-AP | Download protocol |
IP protocol for SIN3A antibody 14638-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma. | ||
Int J Biol Sci TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis. | ||
Cell Rep ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression | ||
Nat Commun Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages | ||
Bioact Mater Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth |