Histone H4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Histone H4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

63

Cat no : 16047-1-AP

Synonyms

HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3

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Validation Data Gallery

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  WB analysis using 16047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16047-1-AP

  WB result of Histone H4 antibody (16047-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H4 transfected HEK-293 cells.

• 

  WB analysis of MCF-7 using 16047-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16047-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16047-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16047-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16047-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16047-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat thymus using 16047-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16047-1-AP

  IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.

• 

  IP experiment of HeLa using 16047-1-AP

  IP result of anti-Histone H4 (IP:16047-1-AP, 4ug; Detection:16047-1-AP 1:300) with HeLa cells lysate 2680ug.

• 

  IHC staining of mouse small intestine using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16047-1-AP

  Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16047-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 16047-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16047-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  ChIP experiment of HEK-293 using 16047-1-AP

  Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, HT-1080 cells, MCF-7 cells, mouse kidney tissue, mouse thymus tissue, rat thymus tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse small intestine tissue, mouse colon tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells
• Positive ChIP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprecipitation (IP): CHIP :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 55 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Product Information

16047-1-AP targets Histone H4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H4 fusion protein Ag8999
• Full Name: histone cluster 1, H4e
• Calculated Molecular Weight: 102 aa, 11 kDa
• Observed Molecular Weight: 14 kDa, 11 kDa
• GenBank Accession Number: BC012587
• Gene Symbol: Histone H4
• Gene ID (NCBI): 8367
• RRID: AB_2118625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Publications

Species	Application	Title
mouse	WB	Cell; Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.; Authors - Lilien N Voong ; KD Validated
human	WB	Cell Res; Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.; Authors - Xianteng Wang
mouse	WB	Cell Res; SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation.; Authors - Fang Yu
rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng
human,mouse	IHC	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans

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