Histone H3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Histone H3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

939

Reviews (9)

Cat no : 17168-1-AP

Synonyms

HIST2H3A, H3-clustered histone 15, H3-clustered histone 14, H3-clustered histone 13, H3C15

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 17168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone H3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17168-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17168-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 17168-1-AP

  IP result of anti-Histone-H3 (IP:17168-1-AP, 4ug; Detection:17168-1-AP 1:8000) with MCF-7 cells lysate 2120 ug.

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  IHC staining of human oesophagus cancer using 17168-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone H3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 17168-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone H3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17168-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 17168-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 17168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17168-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17168-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H3 antibody (17168-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

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  IF Staining of HeLa using 17168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 17168-1-AP

  Immunofluorescent analysis of HeLa cells using 17168-1-AP (Histone-H3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 17168-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Histone H3 Polyclonal antibody (17168-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, mouse skeletal muscle tissue, mouse liver tissue, mouse brain tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human oesophagus cancer tissue, human skin cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 882 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 9 publications below

Product Information

17168-1-AP targets Histone H3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, goat, fish, arabidopsis, yellow catfish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H3 fusion protein Ag10644
• Full Name: histone cluster 2, H3a
• Calculated Molecular Weight: 136 aa, 15 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC015544
• Gene Symbol: Histone H3
• Gene ID (NCBI): 333932
• RRID: AB_2716755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Publications

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
human	WB	Nature; The molecular basis of tight nuclear tethering and inactivation of cGAS.; Authors - Baoyu Zhao
	WB	Nature; A phase-separated nuclear GBPL circuit controls immunity in plants.; Authors - Shuai Huang
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (08-19-2024)

Worked on blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: IMCD3

FH

Wissem (Verified Customer) (06-27-2024)

Antibody works very well at detecting Histone H3 in extracts from U2OS, HCT116, HeLa, RPE1 and HEK293 cells - used at 1:10,000 to 1:15,000 incubated overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000

FH

Roy (Verified Customer) (06-12-2024)

This antibody is great at detecting Histone H3 by WB (1/1000- 1h incubation at RT) with beautiful enrichment at the purified chromatin fraction.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa cells

FH

Mi (Verified Customer) (06-25-2023)

It works very well in nuclear extracts from human brown adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: human brown adipocytes

FH

Veda (Verified Customer) (04-29-2022)

IF staining test in cryo sections of skin. It does not seem to work. Maybe it only stains the nuclear membrane.

• Applications: Immunofluorescence

FH

Pradeep (Verified Customer) (09-19-2020)

Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart Tissue

FH

Tanusree (Verified Customer) (12-03-2019)

The antibody works great in Western blotting analysis.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Ruiting (Verified Customer) (11-11-2019)

I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse testis and brains

FH

Robert (Verified Customer) (07-18-2019)

The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

Histone H3 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:17168-1-AP)

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