Anticorps Polyclonal de lapin anti-MED15

• Phare
• Validé par KD/KO

MED15 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 11566-1-AP

Synonymes

ARC105, CAG7A, CTG repeat protein 7a, CTG7A, MED15, mediator complex subunit 15, PCQAP, TIG 1, TIG1, TNRC7, TPA inducible gene 1 protein

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Galerie de données de validation

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  WB analysis of K-562 using 11566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 11566-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 11566-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11566-1-AP

  IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug.

• 

  IHC staining of human prostate cancer using 11566-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 11566-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules SMMC-7721
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• ChIP: See 4 publications below

Informations sur le produit

11566-1-AP cible MED15 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED15 Protéine recombinante Ag2143
• Nom complet: mediator complex subunit 15
• Masse moléculaire calculée: 788 aa, 87 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC017110
• Symbole du gène: MED15
• Identification du gène (NCBI): 51586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

Publications

Species	Application	Title
		Mol Cell; Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.; Authors - Nilay Shah
mouse	WB	Cell Rep; Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.; Authors - Min Xia
human	WB,IHC,IF	Int J Cancer; MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.; Authors - Zaki Shaikhibrahim ; KD Validated
human, mouse	ChIP	PLoS Genet; Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.; Authors - Naomi Shomer
human	WB	J Biol Chem; Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes.; Authors - Tsutsui Taiki T
human	WB, IHC, IF	Am J Pathol; Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma.; Authors - Zaki Shaikhibrahim

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

between 75-100

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED15 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:11566-1-AP)

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