TRIM11 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10851-1-AP

Synonyms

BIA1, Protein BIA1, RING finger protein 92, RNF92, TRIM11, tripartite motif containing 11

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Validation Data Gallery

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  WB analysis using 10851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10851-1-AP (TRIM11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• CoIP: See 5 publications below

Product Information

10851-1-AP targets TRIM11 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM11 fusion protein Ag1308
• Full Name: tripartite motif-containing 11
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011629
• Gene Symbol: TRIM11
• Gene ID (NCBI): 81559
• RRID: AB_2209210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM11(Tripartite motif-containing protein 11) is also named as RNF92 and Belongs to the TRIM/RBCC family. It is a member of the tripartite-motif-containing protein family and is known to destabilize humanin, an inhibitor of Alzheimer-like neuronal insults(PMID: 16904669). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
		Science; TRIM11 protects against tauopathies and is down-regulated in Alzheimer's disease; Authors - Zi-Yang Zhang
mouse	WB,IF,CoIP	J Hazard Mater; The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM2.5 exposure-induced pulmonary fibrosis of mice.; Authors - Yaling Zhang
human	IHC,IF	Oncogene; TRIM11 is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth.; Authors - Di K K
mouse	WB,IHC	FASEB J; The topological key lncRNA H2k2 from the ceRNA network promotes mesangial cell proliferation in diabetic nephropathy via the miR-449a/b/Trim11/Mek signaling pathway.; Authors - Wenyun Chen
human	IF,CoIP, WB	Int J Biol Sci; SOX13/TRIM11/YAP axis promotes the proliferation, migration and chemoresistance of anaplastic thyroid cancer.; Authors - Jianing Tang
human	WB,IF,CoIP	Neoplasia; TRIM11 promotes breast cancer cell proliferation by stabilizing estrogen receptor α.; Authors - Jianing Tang ; KD Validated