PLAGL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLAGL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11540-1-AP

Synonyms

KIAA0198, PLAGL2, Zinc finger protein PLAGL2

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Validation Data Gallery

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  WB analysis using 11540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11540-1-AP (PLAGL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

11540-1-AP targets PLAGL2 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLAGL2 fusion protein Ag2137
• Full Name: pleiomorphic adenoma gene-like 2
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa, 70 kDa
• GenBank Accession Number: BC023655
• Gene Symbol: PLAGL2
• Gene ID (NCBI): 5326
• RRID: AB_2165035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).

Publications

Species	Application	Title
human	WB,IHC,IF	EBioMedicine; PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.; Authors - Daojiang Li ; KD Validated
human	IP	Cancers (Basel); Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.; Authors - Qinghua Wang
human,mouse	WB,IHC,IF	Carcinogenesis; PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis; Authors - Wen Chen
human	WB	Apoptosis; Pleomorphic adenoma gene-like 2 regulates expression of the p53 family member, p73, and induces cell cycle block and apoptosis in human promonocytic U937 cells.; Authors - Hanks Tracey S TS
human	WB,IHC	Pediatr Res; Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2.; Authors - Liang Wu ; KD Validated
human	WB,IF	Gene; Modulation of PLAGL2 transactivation by positive cofactor 2 (PC2), a component of the ARC/Mediator complex.; Authors - Wezensky Sara J SJ