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  • KD/KO Validated

MED12 Polyclonal antibody

MED12 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20028-1-AP


ARC240, CAG repeat protein 45, CAGH45, FGS1, HOPA, KIAA0192, MED12, mediator complex subunit 12, OKS, OPA containing protein, OPA1, TNRC11, TRAP230

Tested Applications

Positive WB detected inmouse liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20028-1-AP targets MED12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mediator complex subunit 12
Calculated Molecular Weight 243 kDa
Observed Molecular Weight 243 kDa
GenBank Accession NumberNM_005120
Gene Symbol MED12
Gene ID (NCBI) 9968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.


Product Specific Protocols
WB protocol for MED12 antibody 20028-1-APDownload protocol
IF protocol for MED12 antibody 20028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang

Mod Pathol

MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.

Authors - Elizabeth Bertsch

Genes (Basel)

MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.

Authors - Yingchao Zhou
  • KD Validated

Pathol Res Pract

Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma.

Authors - Nobuko Yasutake