MED12 Polyclonal antibody

• Featured Product
• KD/KO Validated

MED12 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20028-1-AP

Synonyms

HOPA, FGS1, CAGH45, CAG repeat protein 45, ARC240

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Validation Data Gallery

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  WB analysis of mouse liver using 20028-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20028-1-AP (MED12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20028-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20028-1-AP (MED12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20028-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 20028-1-AP (MED12 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

20028-1-AP targets MED12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mediator complex subunit 12
• Calculated Molecular Weight: 243 kDa
• Observed Molecular Weight: 243 kDa
• GenBank Accession Number: NM_005120
• Gene Symbol: MED12
• Gene ID (NCBI): 9968
• RRID: AB_10644141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.

Publications

Species	Application	Title
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	IHC,IF	Mod Pathol; MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.; Authors - Elizabeth Bertsch
human,mouse	WB,IHC,IF	Genes (Basel); MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.; Authors - Yingchao Zhou ; KD Validated
human	IHC	Pathol Res Pract; Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma.; Authors - Nobuko Yasutake
human	IF	Naunyn Schmiedebergs Arch Pharmacol; Loureirin B improves H/R-induced hepatic ischemia-reperfusion injury by downregulating ALOX5 to regulate mitochondrial homeostasis; Authors - Zhaolong Yu