- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-MDM2
MDM2 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IF, IHC, COIP, ELISA
Conjugaison
Non conjugué
N° de cat : 19058-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules A431, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 2 publications below |
| WB | See 49 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
19058-1-AP cible MDM2 dans les applications de WB, IP, IF, IHC, COIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | Mdm2 p53 binding protein homolog (mouse) |
| Masse moléculaire calculée | 55 kDa |
| Poids moléculaire observé | 30-55 kDa, 60-100 kDa |
| Numéro d’acquisition GenBank | NM_002392 |
| Symbole du gène | MDM2 |
| Identification du gène (NCBI) | 4193 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
What is the cellular localization of MDM2?
MDM2 is predominantly localized in the nucleoplasm.
What is the tissue specificity of MDM2?
MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain
isoforms only express in cancerous cells and not normal tissue.
With what other molecules does MDM2 interact?
Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional
activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to
MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.
What competes with MDM2 activity?
Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.
What is the role of ubiquitination in MDM2 activity?
While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination
mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of
p53.
How is MDM2 associated with cancer?
MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in
promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development
and growth.
What is the human ortholog of MDM2?
Human double-minute type 2 (HDM2)
In what cellular circumstances does the p53-MDM2 complex dissociate?
Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also
prevents p53-MDM2 interaction.
Where is the gene located?
Hdm2 is located on human 12q15.
Mdm2 is located on murine 10 D2.
PMIDs: 24309898, 29584707, 9840926, 28714398
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for MDM2 antibody 19058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A). | ||
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Theranostics Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells. | ||
Clin Transl Med Genome-wide CRISPR/Cas9 screening for therapeutic targets in NSCLC carrying wild-type TP53 and receptor tyrosine kinase genes. | ||
Redox Biol Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH balawant (Verified Customer) (08-23-2022) | i will recommend this antibody for westernblot. it is working good
|
FH Wei (Verified Customer) (01-30-2020) | Weak band for mouse heart at basal state.
|
