RPL11 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL11 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 16277-1-AP

Synonyms

60S ribosomal protein L11, CLL associated antigen KW 12, DBA7, GIG34, ribosomal protein L11, RPL11

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Validation Data Gallery

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  WB analysis of human liver using 16277-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16277-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 16277-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16277-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16277-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16277-1-AP (RPL11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16277-1-AP (RPL11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 16277-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16277-1-AP (RPL11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

16277-1-AP targets RPL11 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL11 fusion protein Ag9356
• Full Name: ribosomal protein L11
• Calculated molecular weight: 177 aa, 20 kDa
• Observed molecular weight: 20 kDa
• GenBank accession number: BC018970
• Gene symbol: RPL11
• Gene ID (NCBI): 6135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.

Publications

Species	Application	Title
human	WB,IF	Oxid Med Cell Longev; BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis.; Authors - Fangyuan Jia ; KD Validated
human	WB	Cells; Knockdown of the Ribosomal Protein eL29 in Mammalian Cells Leads to Significant Changes in Gene Expression at the Transcription Level.; Authors - Alexander V Gopanenko
human	WB	Int J Mol Sci; Deficiency of the Ribosomal Protein uL5 Leads to Significant Rearrangements of the Transcriptional and Translational Landscapes in Mammalian Cells.; Authors - Elena S Babaylova ; KD Validated
human	IP	PLoS One; Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.; Authors - Robin Assfalg
human	WB,IF,CoIP	Int J Oncol; Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.; Authors - Runze Wang ; KD Validated
human	WB	DNA Repair (Amst); Regulation of Actinomycin D induced upregulation of Mdm2 in H1299 cells.; Authors - Li Lianjie L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anita (Verified Customer) (12-22-2020)

Works for C. elegans at a protein concentration of 5 micrograms and above

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans

RPL11 Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:16277-1-AP)

FH

Zehua (Verified Customer) (12-17-2019)

It works excellent for WB analysis. I strongly suggested this antibody.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell lines

RPL11 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in Cell lines (Cat no:16277-1-AP)

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