Tested Applications
| Positive WB detected in | BxPC-3 cells, human liver tissue, mouse lung tissue, mouse pancreas tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16277-1-AP targets RPL11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9356 Product name: Recombinant human RPL11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-177 aa of BC018970 Sequence: MADQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK Predict reactive species |
| Full Name | ribosomal protein L11 |
| Calculated Molecular Weight | 177 aa, 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC018970 |
| Gene Symbol | RPL11 |
| Gene ID (NCBI) | 6135 |
| RRID | AB_2181292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62913 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL11 antibody 16277-1-AP | Download protocol |
| IHC protocol for RPL11 antibody 16277-1-AP | Download protocol |
| WB protocol for RPL11 antibody 16277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RPL11 Polyclonal antibody (Cat# 16277-1-AP) has accumulated 44 citations across journals including Science, Cell, Nature Communications, Molecular Cell, Cell Reports, Signal Transduction and Targeted Therapy, Cancer Research, Blood, Developmental Cell, Oncogene, and others. Associated research fields include ribosome biogenesis, p53/MDM2 signaling, hepatocellular carcinoma, glioblastoma, lung cancer, RNA processing, translation regulation, ferroptosis, nucleolar stress, autophagy, and spermiogenesis.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma | ||
Signal Transduct Target Ther A ribosomal gene panel predicting a novel synthetic lethality in non-BRCAness tumors
| ||
Science Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes | ||
Cell A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence | ||
Cell Mol Immunol Dhx33 promotes B-cell growth and proliferation by controlling activation-induced rRNA upregulation | ||
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution (lot 00023669). One user reported it works well for C. elegans at protein concentrations of 5 µg or above, rating it 4 out of 5 stars. The other user found it excellent for WB analysis in cell lines and strongly recommended the antibody, giving it a perfect 5-star rating. Overall, users are satisfied with its performance in Western Blot applications across different species and sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anita (Verified Customer) (12-22-2020) | Works for C. elegans at a protein concentration of 5 micrograms and above
 |
Zehua (Verified Customer) (12-17-2019) | It works excellent for WB analysis. I strongly suggested this antibody.
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