RPL11 Polyclonal antibody

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  WB analysis of human liver using 16277-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 16277-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 16277-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16277-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16277-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16277-1-AP (RPL11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16277-1-AP (RPL11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 16277-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using RPL11 antibody (16277-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

RPL11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16277-1-AP

Publications(34)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

60S ribosomal protein L11, CLL associated antigen KW 12, CLL-associated antigen KW-12, DBA7, GIG34

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Tested Applications

• Positive WB detected in: human liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 31 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16277-1-AP targets RPL11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL11 fusion protein Ag9356
• Full Name: ribosomal protein L11
• Calculated Molecular Weight: 177 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC018970
• Gene Symbol: RPL11
• Gene ID (NCBI): 6135
• RRID: AB_2181292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62913
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.

Publications

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	WB	Am J Hum Genet; Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.; Authors - Koji Nakamura
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anita (Verified Customer) (12-22-2020)

Works for C. elegans at a protein concentration of 5 micrograms and above

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans

RPL11 Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:16277-1-AP)

FH

Zehua (Verified Customer) (12-17-2019)

It works excellent for WB analysis. I strongly suggested this antibody.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell lines

RPL11 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in Cell lines (Cat no:16277-1-AP)