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  • KD/KO Validated

USP7 Polyclonal antibody

USP7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26948-1-AP

Synonyms

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7



Tested Applications

Positive WB detected inMCF-7 cells, U2OS cells, K-562 cells, mouse spleen tissue, NIH/3T3 cells, PC-12 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26948-1-AP targets USP7 in WB, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP7 fusion protein Ag25634
Full Name ubiquitin specific peptidase 7 (herpes virus-associated)
Observed Molecular Weight 126-128 kDa
Gene Symbol USP7
Gene ID (NCBI) 7874
RRIDAB_2880696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for USP7 antibody 26948-1-APDownload protocol
IHC protocol for USP7 antibody 26948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cancer Res

Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.

Authors - Monika Vishnoi
humanWB

Front Immunol

Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.

Authors - Wenying Gao
  • KD Validated
humanWB,IHC

Int J Biol Sci

Flap endonuclease 1 Facilitated Hepatocellular Carcinoma Progression by Enhancing USP7/MDM2-mediated P53 Inactivation.

Authors - Saiyan Bian
humanWB

Discov Oncol

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer

Authors - Haiqing Wang
mouseWB,IHC

Front Pharmacol

Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice

Authors - Yu-Hui Gu
humanWB

ACS Pharmacol Transl Sci

Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf

Authors - Hongyue Li