AKT Monoclonal antibody

• Featured Product
• KD/KO Validated

AKT Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

484

CloneNo.

2C5D1

Reviews (1)

Cat no : 60203-2-Ig

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT

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Validation Data Gallery

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  WB analysis using 60203-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60203-2-Ig

  WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells.

• 

  WB analysis of HEK-293 using 60203-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60203-2-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60203-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60203-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60203-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 60203-2-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60203-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60203-2-Ig

  IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 60203-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, rat liver tissue, mouse brain tissue, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells, LNCaP cells, Hela cells, Jurkat cells, HSC-T6 cells, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 472 publications below
• IHC: See 14 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

60203-2-Ig targets AKT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, chicken, bovine, hamster, sheep, duck
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AKT fusion protein Ag16695
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56-62 kDa
• GenBank Accession Number: BC000479
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• RRID: AB_10912803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Commun; FOXP3+ regulatory T cell perturbation mediated by the IFNγ-STAT1-IFITM3 feedback loop is essential for anti-tumor immunity; Authors - Xinnan Liu
mouse	WB	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv
human	WB	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu
mouse,rat	WB	Toxicology; Protective role of cezanne in doxorubicin-induced cardiotoxicity by inhibiting autophagy, apoptosis and oxidative stress; Authors - Jiayan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Janine (Verified Customer) (02-18-2019)

Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RPE-1 cell lysates (6cm dish)

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