• Featured Product
  • KD/KO Validated

AKT Monoclonal antibody

AKT Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, CoIP, ELISA, IF/ICC

Conjugate

Unconjugated

CloneNo.

2C5D1

Cat no : 60203-2-Ig

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT



Tested Applications

Positive WB detected inA549 cells, rat liver tissue, mouse brain tissue, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells, LNCaP cells, Hela cells, Jurkat cells, HSC-T6 cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60203-2-Ig targets AKT in WB, IP, IHC, CoIP, ELISA, IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, sheep, rabbit, hamster, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKT fusion protein Ag16695
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_10912803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 60203-2-IgDownload protocol
IHC protocol for AKT antibody 60203-2-IgDownload protocol
IF protocol for AKT antibody 60203-2-IgDownload protocol
IP protocol for AKT antibody 60203-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

FOXP3+ regulatory T cell perturbation mediated by the IFNγ-STAT1-IFITM3 feedback loop is essential for anti-tumor immunity

Authors - Xinnan Liu
mouseWB

Cell Rep Med

Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing

Authors - Dongming Lv
humanWB

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
mouseWB

Research (Wash D C)

Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis

Authors - Lang Linghu
mouse,ratWB

Toxicology

Protective role of cezanne in doxorubicin-induced cardiotoxicity by inhibiting autophagy, apoptosis and oxidative stress

Authors - Jiayan Zhang
ratWB

Acta Biomater

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Authors - Shanshan Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Janine (Verified Customer) (02-18-2019)

Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: RPE-1 cell lysates (6cm dish)