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  • KD/KO Validated

MDMX Polyclonal antibody

MDMX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17914-1-AP


Double minute 4 protein, HDMX, Mdm2 like p53 binding protein, MDM4, MDMX, MRP1, p53 binding protein Mdm4, Protein Mdm4, Protein Mdmx

Tested Applications

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17914-1-AP targets MDMX in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDMX fusion protein Ag12319
Full Name Mdm4 p53 binding protein homolog (mouse)
Calculated Molecular Weight 490 aa, 55 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC067299
Gene Symbol MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for MDMX antibody 17914-1-APDownload protocol
IHC protocol for MDMX antibody 17914-1-APDownload protocol
IP protocol for MDMX antibody 17914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Authors - Hyojung Kim

Cancer Res

FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.

Authors - Xiang Ling

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang

Cell Death Discov

LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

Authors - Qingqing Zhao

J Med Chem

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.

Authors - Shiyan Zhang

PLoS Genet

MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.

Authors - Meenalakshmi Chinnam
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Breanna (Verified Customer) (02-18-2020)

It works well

  • Applications: ChIP,

Breanna (Verified Customer) (01-29-2020)

Works well

  • Applications: Immunoprecipitation, Flow Cytometry,