Anticorps Polyclonal de lapin anti-Myelin basic protein

Myelin basic protein Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 10458-1-AP

Synonymes

MBP, Myelin A1 protein, Myelin basic protein



Applications testées

Résultats positifs en WBtissu cérébral de souris, tissu cérébral de rat
Résultats positifs en IHCtissu cérébral de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu cérébral de souris,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10458-1-AP cible Myelin basic protein dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Myelin basic protein Protéine recombinante Ag0713
Nom complet myelin basic protein
Masse moléculaire calculée 33 kDa
Poids moléculaire observé 14-23 kDa
Numéro d’acquisition GenBankBC008749
Symbole du gène Myelin basic protein
Identification du gène (NCBI) 4155
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

Protocole

Product Specific Protocols
WB protocol for Myelin basic protein antibody 10458-1-APDownload protocol
IHC protocol for Myelin basic protein antibody 10458-1-APDownload protocol
IF protocol for Myelin basic protein antibody 10458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

ACS Nano

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia

Authors - Shishi Shen
ratIF

ACS Nano

Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury

Authors - Weidong Wu
human,mouseIF

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
ratIF

Microbiome

The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.

Authors - Weiping Xiao
mouseIHC

Brain

Sensory neurons have an axon initial segment that initiates spontaneous activity in neuropathic pain.

Authors - Ana I Nascimento
mouseIHC

J Pineal Res

Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors

Authors - Yao Zuo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kenzo (Verified Customer) (08-16-2023)

This MBP antibody worked well and generated robust immunofluorescence signals on mouse optic nerve sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse optic nerve
FH

Rebecca (Verified Customer) (02-10-2022)

Western performed on Biorad MiniPROTEAN 4-15% TGX precast gels and Precision Plus All Blue Protein Ladder and 4x Laemmli Sample Buffer. Blocking performed for 1h at room temperature using 5%BLOTTO. Primary antibody dilution was performed overnight at 4°C in 5%BLOTTO/0.05%Tween. Secondary antibody was LiCOR IRDye® 680RD Donkey anti-Mouse IgG (H + L) at 1:20000 dilution in PBS-Tween-SDS as per manufacturers instructions for 2h at room temperature. Blot showed clear bands of the expected isoform sizes with very low-none background/off target binding.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Rat brain whole tissue lysate (50ug)
Myelin basic protein Antibody Western Blot validation (1:5000 dilution) in Rat brain whole tissue lysate (50ug) (Cat no:10458-1-AP)