PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 19132-1-AP

Synonyms

MAY1, PKC delta, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

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Validation Data Gallery

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  WB analysis using 19132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19132-1-AP

  Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

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  WB analysis of Jurkat using 19132-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19132-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 19132-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19132-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 19132-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 19132-1-AP

  IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug.

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  IP experiment of A431 using 19132-1-AP

  IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug.

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  IHC staining of human brain using 19132-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 19132-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 19132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 19132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of U2OS using 19132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U-251 using 19132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 19132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 19132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 19132-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, MCF-7 cells, HT-1080 cells, mouse colon tissue, HUVEC cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human brain tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells, U-251 cells, HeLa cells, A431 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

19132-1-AP targets PKC Delta in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Delta fusion protein Ag5631
• Full Name: protein kinase C, delta
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC043350
• Gene Symbol: PRKCD
• Gene ID (NCBI): 5580
• RRID: AB_10637263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Virol Sin; Rottlerin plays an antiviral role at early and late steps of Zika virus infection; Authors - Shili Zhou ; KD Validated
rat	WB	Food Chem Toxicol; G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.; Authors - Yao Liu
	WB	Fish Shellfish Immunol; In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.; Authors - Zhong-Wen Chang
mouse	WB	iScience; Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli.; Authors - Houbao Qi
mouse	WB	Neurochem Int; Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.; Authors - Katarzyna Dąbrowska
rat	WB	J Ethnopharmacol; Tetrastigma hemsleyanum suppresses neuroinflammation in febrile seizures rats via regulating PKC-δ/caspase-1 signaling pathway; Authors - Weiwei Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

B (Verified Customer) (07-11-2022)

A good antibody for WB with the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PKC Delta Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:19132-1-AP)