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  • KD/KO Validated

PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19132-1-AP


MAY1, PKC delta, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, MCF-7 cells, HT-1080 cells, mouse colon tissue, HUVEC cells
Positive IP detected inA431 cells
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, U-251 cells, HeLa cells, A431 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19132-1-AP targets PKC Delta in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Delta fusion protein Ag5631
Full Name protein kinase C, delta
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC043350
Gene Symbol PRKCD
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for PKC Delta antibody 19132-1-APDownload protocol
IHC protocol for PKC Delta antibody 19132-1-APDownload protocol
IF protocol for PKC Delta antibody 19132-1-APDownload protocol
IP protocol for PKC Delta antibody 19132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Rottlerin plays an antiviral role at early and late steps of Zika virus infection

Authors - Shili Zhou
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Food Chem Toxicol

G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.

Authors - Yao Liu

Fish Shellfish Immunol

In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.

Authors - Zhong-Wen Chang


Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli.

Authors - Houbao Qi

Neurochem Int

Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.

Authors - Katarzyna Dąbrowska

J Ethnopharmacol

Tetrastigma hemsleyanum suppresses neuroinflammation in febrile seizures rats via regulating PKC-δ/caspase-1 signaling pathway

Authors - Weiwei Ji


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


B (Verified Customer) (07-11-2022)

A good antibody for WB with the right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
PKC Delta Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:19132-1-AP)