PRMT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 11279-1-AP

Synonyms

Interferon receptor 1-bound protein 4, HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319

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Validation Data Gallery

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11279-1-AP

  WB result of PRMT1 antibody (11279-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT1 transfected A549 cells.

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11279-1-AP

  IP result of anti-PRMT1 (IP:11279-1-AP, 4ug; Detection:11279-1-AP 1:2000) with A549 cells lysate 920 ug.

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  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 11279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (11279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11279-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRMT1 Polyclonal antibody (11279-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Product Information

11279-1-AP targets PRMT1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRMT1 fusion protein Ag1790
• Full Name: protein arginine methyltransferase 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC019268
• Gene Symbol: PRMT1
• Gene ID (NCBI): 3276
• RRID: AB_2171319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li
mouse	WB,IP	Nucleic Acids Res; RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility; Authors - Heejin Choi
human	WB	Cell Death Dis; PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis; Authors - Hao Liu
human	IHC	Oncogene; PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.; Authors - Xin-Ke Yin
human,mouse	WB,IHC	NPJ Precis Oncol; The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer; Authors - Xiangwei Zeng
mouse	WB	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs