- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-MAP4
MAP4 Polyclonal Antibody for WB, IP, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IF, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 11229-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules C2C12, cellules C6, cellules HEK-293, cellules HepG2 |
Résultats positifs en IP | cellules HEK-293 |
Résultats positifs en IHC | tissu de cancer de l'œsophage humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 4 publications below |
WB | See 12 publications below |
IHC | See 6 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Informations sur le produit
11229-1-AP cible MAP4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | MAP4 Protéine recombinante Ag1741 |
Nom complet | microtubule-associated protein 4 |
Masse moléculaire calculée | 121 kDa |
Poids moléculaire observé | 210-240 kDa |
Numéro d’acquisition GenBank | BC012794 |
Symbole du gène | MAP4 |
Identification du gène (NCBI) | 4134 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)
Protocole
Product Specific Protocols | |
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WB protocol for MAP4 antibody 11229-1-AP | Download protocol |
IHC protocol for MAP4 antibody 11229-1-AP | Download protocol |
IP protocol for MAP4 antibody 11229-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Neuropathol Divergent brain gene expression patterns associate with distinct cell-specific tau neuropathology traits in progressive supranuclear palsy. | ||
Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. | ||
J Mol Cell Biol The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability. | ||
Oncogene Microtubule-associated protein 4 is an important regulator of cell invasion/migration and a potential therapeutic target in esophageal squamous cell carcinoma.
| ||
J Innate Immun Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis | ||
Front Cell Infect Microbiol Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alice (Verified Customer) (08-09-2022) | Not bad for western blot. A bit faint but had bands in expected places and can see clear data.
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FH CHAO (Verified Customer) (03-31-2022) | Multiple bands may be due to different post-translational modifications
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