• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-MAP4

MAP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 11229-1-AP

Synonymes

MAP 4, MAP4



Applications testées

Résultats positifs en WBcellules HeLa, cellules C2C12, cellules C6, cellules HEK-293, cellules HepG2
Résultats positifs en IPcellules HEK-293
Résultats positifs en IHCtissu de cancer de l'œsophage humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

11229-1-AP cible MAP4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MAP4 Protéine recombinante Ag1741
Nom complet microtubule-associated protein 4
Masse moléculaire calculée 121 kDa
Poids moléculaire observé 210-240 kDa
Numéro d’acquisition GenBankBC012794
Symbole du gène MAP4
Identification du gène (NCBI) 4134
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

Protocole

Product Specific Protocols
WB protocol for MAP4 antibody 11229-1-APDownload protocol
IHC protocol for MAP4 antibody 11229-1-APDownload protocol
IP protocol for MAP4 antibody 11229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Acta Neuropathol

Divergent brain gene expression patterns associate with distinct cell-specific tau neuropathology traits in progressive supranuclear palsy.

Authors - Mariet Allen
humanWB

Brain

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Authors - Janja Božič
humanWB,IF

J Mol Cell Biol

The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability.

Authors - Yuan Wu
humanWB

Oncogene

Microtubule-associated protein 4 is an important regulator of cell invasion/migration and a potential therapeutic target in esophageal squamous cell carcinoma.

Authors - Y-Y Jiang
  • KO Validated
mouseWB

J Innate Immun

Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis

Authors - Xiaogang Luo
humanWB

Front Cell Infect Microbiol

Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6.

Authors - Jie Zan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alice (Verified Customer) (08-09-2022)

Not bad for western blot. A bit faint but had bands in expected places and can see clear data.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Sheep atrial tissue
MAP4 Antibody Western Blot validation (1:1000 dilution) in Sheep atrial tissue (Cat no:11229-1-AP)
FH

CHAO (Verified Customer) (03-31-2022)

Multiple bands may be due to different post-translational modifications

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart