- Featured Product
- KD/KO Validated
Cyclin B1 Polyclonal antibody
Cyclin B1 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Cat no : 55004-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells
|Positive IP detected in
|Positive IF detected in
|HeLa cells, U2OS cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55004-1-AP targets Cyclin B1 in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, sheep, bovine
|Host / Isotype
|Rabbit / IgG
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
Signal Transduct Target Ther
MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
Acta Pharm Sin B
Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.
Acta Pharm Sin B
The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.
Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.
PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.
Cell Death Dis
HDAC7/c-Myc signaling pathway promotes the proliferation and metastasis of choroidal melanoma cells