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Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (4)





Cat no : 55004-1-AP


CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells, U2OS cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55004-1-AP targets Cyclin B1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, sheep, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin B1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberNM_031966
Gene Symbol Cyclin B1
Gene ID (NCBI) 891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.


Product Specific Protocols
WB protocol for Cyclin B1 antibody 55004-1-APDownload protocol
IF protocol for Cyclin B1 antibody 55004-1-APDownload protocol
IP protocol for Cyclin B1 antibody 55004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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