Beta Tubulin Monoclonal antibody
Beta Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, nematode, pig, zebrafish and More (4)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
375
CloneNo.
1D4A4
Cat no : 66240-1-Ig
Synonyms
"Beta Tubulin Antibodies" Comparison
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells, ROS1728 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human breast cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 341 publications below |
| IHC | See 1 publications below |
| IF | See 24 publications below |
| IP | See 3 publications below |
Product Information
66240-1-Ig targets Beta Tubulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, nematode, pig, zebrafish samples.
| Tested Reactivity | human, mouse, rat, nematode, pig, zebrafish |
| Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, chicken, zebrafish, sheep |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Beta Tubulin fusion protein Ag0117 |
| Full Name | tubulin, beta 3 |
| Calculated Molecular Weight | 450 aa, 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC000748 |
| Gene Symbol | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2881629 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.
What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?
The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).
How to use beta-tubulin as a loading control
Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.
What drugs can influence beta-tubulin and organization of microtubules?
Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.
Is beta-tubulin post-translationally modified?
Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IHC protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IF protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IP protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Science A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements. | ||
Adv Mater A Physiologically Responsive Nanocomposite Hydrogel for Treatment of Head and Neck Squamous Cell Carcinoma via Proteolysis-targeting Chimeras Enhanced Immunotherapy | ||
Gut Integrative multiomics enhancer activity profiling identifies therapeutic vulnerabilities in cholangiocarcinoma of different etiologies |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH P (Verified Customer) (09-23-2024) | Excellent
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FH Moritz (Verified Customer) (09-12-2024) | The antibody worked very well and gave a very strong signal (can be diluted more). Used it in HEK293T cell lysate analysed with WB.
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FH Tsimafei (Verified Customer) (08-04-2024) | Excellent antibody! The membrane was incubated for 1h at RT
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FH Andrea (Verified Customer) (01-11-2023) | Antibody were incubated for 2h at room temperature. Provide a specific tubulin signal
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FH Emma (Verified Customer) (11-29-2021) | Very clean antibody and runs at the right size by western blot.
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FH Raul (Verified Customer) (08-20-2021) | Very good antibody producing a single strong band at ~55kDa
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FH Yu Siong (Verified Customer) (08-18-2020) | Western blot of 4 bands: 4 different treatments.
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FH Kathryn (Verified Customer) (12-10-2019) | Used 1:20000 in 5% BSA on a small amount of neonatal mouse ovary. Very clean and specific, and great that it works at such a low concentration.
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FH Sara (Verified Customer) (10-09-2019) | This antibody works great in WB. We use it as loading control in whole cell lysates (mice B cell line), 1:10.000 O/N at 4°C.
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FH VLADIMIR (Verified Customer) (09-25-2019) | B-tubulin IgG2a were used to prepare Fab' conjugated with Alexa 647-maleimide
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FH Jeffery (Verified Customer) (08-01-2019) | Very clean antibody. Works exceptionally well. Runs right at the 50kd marker.
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FH Shashirekha (Verified Customer) (05-09-2019) | Works great!
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FH Zunair (Verified Customer) (12-03-2018) |
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