MAP2 Polyclonal antibody

MAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

275

Reviews (9)

Cat no : 17490-1-AP

Synonyms

Microtubule-associated protein 2, MAP2C, MAP2B, MAP2A, MAP-2

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Validation Data Gallery

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  WB analysis using 17490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17490-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17490-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17490-1-AP

  IP result of anti-MAP2 (IP:17490-1-AP, 3ug; Detection:17490-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IP experiment of mouse brain using 17490-1-AP

  IP result of anti-MAP2 (IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:8000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of SH-SY5Y using 17490-1-AP

  IP result of anti-MAP2 (IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:10000) with SH-SY5Y cells lysate 1240 ug.

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  IHC staining of mouse brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

• 

  IF Staining of mouse brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of iPS cells using 17490-1-AP

  Immunofluorescent staining of MAP2 (17490-1-AP, 1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

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  FC experiment of Neuro-2a using 17490-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (17490-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, rat brain tissue, mouse brain tissue
• Positive IP detected in: SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: iPS cells
• Positive FC (Intra) detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 78 publications below
• IHC: See 31 publications below
• IF: See 188 publications below
• FC: See 1 publications below

Product Information

17490-1-AP targets MAP2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP2 fusion protein Ag11580
• Full Name: microtubule-associated protein 2
• Calculated Molecular Weight: 200 kDa
• Observed Molecular Weight: 280 kDa, 70-85 kDa
• GenBank Accession Number: BC038857
• Gene Symbol: MAP2
• Gene ID (NCBI): 4133
• RRID: AB_2137880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.

What is the molecular weight of MAP2? Is MAP2 post-translationally modified?

MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).

What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?

MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.

Can MAP2 be used as a neuronal marker?

MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).

Publications

Species	Application	Title
mouse	IF	Cell Stem Cell; In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.; Authors - Wenjiao Tai
human	IF	Cell Stem Cell; Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.; Authors - Elke Gabriel
mouse	WB,IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
mouse	WB	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
mouse	IHC,IF	Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Makenna (Verified Customer) (07-15-2024)

Antibody worked well for IF at a dilution of 1:500

• Applications: Immunofluorescence

FH

Abigail (Verified Customer) (07-15-2024)

Used for IF and got clear images in green and red channels.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain cortex

FH

Reyes (Verified Customer) (04-05-2024)

MAP2 (in green) shows a strong marking of my neurons in human brain FFPE cortex

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

MAP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:17490-1-AP)

FH

Filipa (Verified Customer) (09-18-2023)

I used this anti-MAP2 antibody at 1:200 dilution for IF. The cells were fixed in 4% PFA in PBS for 20 minutes, and incubated with the primary antibody for 3 hours at RT. MAP2 staining works very well at this dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: primary rat hippocampal neurons

MAP2 Antibody Immunofluorescence validation (1:200 dilution) in primary rat hippocampal neurons (Cat no:17490-1-AP)

FH

BING (Verified Customer) (08-04-2021)

suitable for detection of both bands of MAP2 by WB with one above 250 kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: primary cortical neuron lysate

MAP2 Antibody Western Blot validation (1:1000 dilution) in primary cortical neuron lysate (Cat no:17490-1-AP)

FH

Dipen (Verified Customer) (07-20-2020)

Not great for Western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Hippocampal neurons

MAP2 Antibody Western Blot, validation (1:500 dilution) in Hippocampal neurons (Cat no:17490-1-AP)

FH

Naomi (Verified Customer) (03-09-2020)

I used the rabbit polyclonal anti-MAP2 antibody at 1:500 dilution to in differentiated PC-12 cells. The cells were fixed in 4% PFA in PBS for 12 minutes. 1 hr in blocking buffer, followed by 24 h incubation in primary antibody. MAP2 was expressed in neurites. Works well at this dilution, great images.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: PC-12

FH

Gloria (Verified Customer) (01-30-2020)

Beautiful staining in primary hippocampal and cortical neurons.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 dilution
• Cell Tissue Type: Primary hippocampal and cortical neurons

MAP2 Antibody Immunofluorescence, validation (1:250 dilution dilution) in Primary hippocampal and cortical neurons (Cat no:17490-1-AP)

FH

Ricky (Verified Customer) (02-22-2019)

1:5000 dilution is good enough for a clear and sharp image of western blot. I am now using Li-Cor's Odyssey CLX imaging systems . The second antibody is from Li-Cor with 1:5000 dilution. By the way, I also tried to use 1:1000 dilution for my low expression samples, and there was no bad noise backgrounds.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

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