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- KD/KO Validated
CDK1-Specific Polyclonal antibody
CDK1-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19532-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells|
|Positive IP detected in||HeLa cells, HEK-293 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
19532-1-AP targets CDK1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||cell division cycle 2, G1 to S and G2 to M|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||NM_001786|
|Gene ID (NCBI)||983|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
|Product Specific Protocols|
|WB protocol for CDK1-Specific antibody 19532-1-AP||Download protocol|
|IHC protocol for CDK1-Specific antibody 19532-1-AP||Download protocol|
|IF protocol for CDK1-Specific antibody 19532-1-AP||Download protocol|
|IP protocol for CDK1-Specific antibody 19532-1-AP||Download protocol|
|FC protocol for CDK1-Specific antibody 19532-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Signal Transduct Target Ther
SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.
Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.
CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.
Shuttling SLC2A4RG is regulated by 14-3-3θ to modulate cell survival via caspase-3 and caspase-6 in human glioma.
Cancer-testis specific gene OIP5: a downstream gene of E2F1 that promotes tumorigenesis and metastasis in glioblastoma by stabilizing E2F1 signaling.