CDK1 Polyclonal antibody

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  WB analysis of HeLa using 19532-1-AP

  WB result of CDK1-Specific antibody (19532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1-Specific transfected HeLa cells.

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  WB analysis using 19532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19532-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19532-1-AP

  IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HeLa cells lysate 1600 ug.

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  IP experiment of HEK-293 using 19532-1-AP

  IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HEK-293 cells lysate 1200 ug.

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  IHC staining of human cervical cancer using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of rat liver using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 19532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1-Specific antibody (19532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 19532-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1-Specific (19532-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

CDK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 19532-1-AP

Publications(221)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CDK1-Specific, CDC2, CDC28A, CDKN1, Cell division protein kinase 1

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells
• Positive IP detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human cervical cancer tissue, mouse liver tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 204 publications below
• IHC: See 21 publications below
• IF: See 9 publications below

Product Information

19532-1-AP targets CDK1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, pig, chicken, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: NM_001786
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• RRID: AB_10638617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo ; KD Validated
human	WB	Nat Cell Biol; Caspase-2 is a condensate-mediated deubiquitinase in protein quality control; Authors - Yingwei Ge
human	IHC	Cell Res; Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.; Authors - Junya Peng
human	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
human	WB	Nat Commun; Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells; Authors - Qiuhong Zhu
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang