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  • KD/KO Validated

CDK1-Specific Polyclonal antibody

CDK1-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey and More (3)





Cat no : 19532-1-AP


CDC2, CDC28A, CDK1, CDK1-Specific, Cell division protein kinase 1, Cyclin dependent kinase 1, p34 protein kinase

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells
Positive IP detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19532-1-AP targets CDK1-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, chicken, sheep, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cell division cycle 2, G1 to S and G2 to M
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberNM_001786
Gene Symbol CDK1
Gene ID (NCBI) 983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.


Product Specific Protocols
WB protocol for CDK1-Specific antibody 19532-1-APDownload protocol
IHC protocol for CDK1-Specific antibody 19532-1-APDownload protocol
IF protocol for CDK1-Specific antibody 19532-1-APDownload protocol
IP protocol for CDK1-Specific antibody 19532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Signal Transduct Target Ther

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Authors - Saisai Guo
  • KD Validated

Cell Res

Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.

Authors - Junya Peng

Nat Commun

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells

Authors - Qiuhong Zhu

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang

Nat Commun

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Authors - Youzhou Sang