Tubulin Beta Class V Monoclonal antibody

Tubulin Beta Class V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, Canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1D7B9

Cat no : 66362-1-Ig

Synonyms

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

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Validation Data Gallery

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  WB analysis of HeLa using 66362-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66362-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66362-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66362-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 66362-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

66362-1-Ig targets Tubulin Beta Class V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, Canine samples.

• Tested Reactivity: human, mouse, Canine
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tubulin, beta 6
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_032525
• Gene Symbol: Beta Tubulin
• Gene ID (NCBI): 84617
• RRID: AB_2881742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.

Publications

Species	Application	Title
mouse	WB	Mediators Inflamm; LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling; Authors - Ling You
rat	WB	Life Sci; NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes.; Authors - Fu Chen
mouse	WB	Dev Neurobiol; Alpha- and beta-tubulin isotypes are differentially expressed during brain development.; Authors - Torben J Hausrat
rat	IHC	BMC Genomics; Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats; Authors - Wang Zhu
mouse	WB	Redox Biol; SENP3 sensitizes macrophages to ferroptosis via de-SUMOylation of FSP1; Authors - Xuelian Chen