Anticorps Polyclonal de lapin anti-Lamin B1

• Phare
• Validé par KD/KO

Lamin B1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

1045

Avis (7)

N° de cat : 12987-1-AP

Synonymes

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 12987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12987-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12987-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 12987-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12987-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 12987-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12987-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12987-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12987-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12987-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12987-1-AP

  IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:20000) with HeLa cells lysate 1560 ug.

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  IHC staining of human liver cancer using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human skin cancer using 12987-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12987-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12987-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 12987-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12987-1-AP

  Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HEK-293 using 12987-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7, cellules SH-SY5Y, cellules U-937, cellules Y79, tissu oculaire de souris, tissu pancréatique de souris, tissu pulmonaire de souris, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer de la peau humain,
• Résultats positifs en IF/ICC: cellules HepG2, cellules NIH/3T3, tissu de cancer de la peau humain
• Résultats positifs en FC (Intra): cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 981 publications below
• IHC: See 9 publications below
• IF: See 55 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

12987-1-AP cible Lamin B1 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, poulet, souris, Hamster, Bombyx Mori
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Lamin B1 Protéine recombinante Ag3631
• Nom complet: lamin B1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC012295
• Symbole du gène: Lamin B1
• Identification du gène (NCBI): 4001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Publications

Species	Application	Title
human	WB,IF	Nat Biotechnol; Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases; Authors - Matthew T N Yarnall
mouse	WB	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
human	IF	Nature; Chromothripsis drives the evolution of gene amplification in cancer.; Authors - Ofer Shoshani
	WB	Nature; Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.; Authors - Yingfeng Li
mouse	WB	Immunity; 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages; Authors - Jun Xiao
human	WB	Cell; The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.; Authors - Jakob Trendel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-21-2023)

Works great in human adipocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes

FH

Ning (Verified Customer) (01-28-2023)

Excellent antibody to probe lamin B1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Lamin B1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:12987-1-AP)

FH

Tsimafei (Verified Customer) (12-04-2022)

20µg of total protein were loaded. Incubation for 2h at room temperature. Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESCs

Lamin B1 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:12987-1-AP)

FH

Alejandro (Verified Customer) (08-07-2022)

Works fine for flow cytometry

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Mouse bone marrow derived macrophages

FH

Iram (Verified Customer) (09-04-2020)

Very sharp bands for nuclear protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

FH

Chun (Verified Customer) (12-05-2019)

Excellent antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: HEK293 and HaCaT cells

FH

Marco (Verified Customer) (09-02-2019)

good staining in conjunction with Alexa Fluor Plus 488 secondary

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human mesenchymal stem cells

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