Tested Applications
| Positive WB detected in | HEK-293 cells, A375 cells, C2C12 cells, HAP1 cells, human brain tissue, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, C6 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12398-1-AP targets LBR in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3088 Product name: Recombinant human LBR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC020079 Sequence: MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSSSPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFG Predict reactive species |
| Full Name | lamin B receptor |
| Calculated Molecular Weight | 615 aa, 71 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC020079 |
| Gene Symbol | LBR |
| Gene ID (NCBI) | 3930 |
| RRID | AB_2138334 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14739 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for LBR antibody 12398-1-AP | Download protocol |
| IF protocol for LBR antibody 12398-1-AP | Download protocol |
| IHC protocol for LBR antibody 12398-1-AP | Download protocol |
| IP protocol for LBR antibody 12398-1-AP | Download protocol |
| WB protocol for LBR antibody 12398-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LBR Polyclonal antibody (Cat# 12398-1-AP) has 15 citations spanning journals including Cell, Nature Cell Biology, Nature Communications, Molecular Cell, Cell Reports, JCI Insight, PLoS Genetics, and others. Research fields covered include nuclear envelope biology, chromatin organization, X-chromosome inactivation, tauopathy and Alzheimer's disease, breast cancer, sperm development, muscular dystrophy, mitochondrial ribosome assembly, DNA damage response, lung cancer, virology, and ischemia-reperfusion injury.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.
| ||
Nat Commun PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. | ||
Mol Cell Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. | ||
J Exp Clin Cancer Res Fast detection, a precise and sensitive diagnostic agent for breast cancer. | ||
Cell Rep The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice. |
