LBR Polyclonal antibody

LBR Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12398-1-AP

Synonyms

DHCR14B, Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta

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Validation Data Gallery

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  WB analysis using 12398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12398-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12398-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 12398-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12398-1-AP

  IP result of anti-LBR (IP:12398-1-AP, 3ug; Detection:12398-1-AP 1:500) with A375 cells lysate 3200ug.

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  IHC staining of human skin cancer using 12398-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12398-1-AP (LBR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12398-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12398-1-AP (LBR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12398-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12398-1-AP (LBR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12398-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (12398-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of C6 using 12398-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 12398-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12398-1-AP

  Immunofluorescent analysis of HepG2 cells, using LBR antibody 12398-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 12398-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LBR (12398-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A375 cells, C2C12 cells, human brain tissue, HeLa cells, HepG2 cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells, C6 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12398-1-AP targets LBR in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LBR fusion protein Ag3088
• Full Name: lamin B receptor
• Calculated Molecular Weight: 615 aa, 71 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC020079
• Gene Symbol: LBR
• Gene ID (NCBI): 3930
• RRID: AB_2138334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

Publications

Species	Application	Title
mouse	IF	Mol Cell; Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy.; Authors - Lulu Jiang
		Nat Commun; PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.; Authors - Chen-Yu Wang
human	WB	PLoS Genet; RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.; Authors - Aurelio Reyes
human	IF	J Cell Sci; Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.; Authors - Janine Pfaff
human	WB,IP	J Cell Mol Med; Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.; Authors - Longfei Li
human	WB,IF	Mol Cell Biol; Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.; Authors - Hongli An