LBR Polyclonal antibody

LBR Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12398-1-AP

Synonyms

DHCR14B, lamin B receptor, LBR, LMN2R, PHA



Tested Applications

Positive WB detected inA375 cells, human brain tissue, C2C12 cells
Positive IP detected inA375 cells
Positive IF detected inHela cells, C6 cells, HepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:25-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12398-1-AP targets LBR in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LBR fusion protein Ag3088
Full Name lamin B receptor
Calculated molecular weight 615 aa, 71 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC020079
Gene symbol LBR
Gene ID (NCBI) 3930
RRIDAB_2138334
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

Protocols

Product Specific Protocols
WB protocol for LBR antibody 12398-1-APDownload protocol
IF protocol for LBR antibody 12398-1-APDownload protocol
IP protocol for LBR antibody 12398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mol Cell

Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy.

Authors - Lulu Jiang

Nat Commun

PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Authors - Chen-Yu Wang
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
humanIF

J Cell Sci

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Authors - Janine Pfaff
humanWB,IP

J Cell Mol Med

Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.

Authors - Longfei Li
humanWB,IF

Mol Cell Biol

Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.

Authors - Hongli An