Lamin B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Lamin B2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 10895-1-AP

Synonyms

LAMB2, lamin B2, LMN2, LMNB2

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Validation Data Gallery

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  WB analysis using 10895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10895-1-AP (LMNB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10895-1-AP

  WB result of Lamin B2 antibody (10895-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B2 transfected HepG2 cells.

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  IP experiment of Jurkat using 10895-1-AP

  IP Result of anti-LMNB2 (IP:10895-1-AP, 3ug; Detection:10895-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human colon cancer using 10895-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10895-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10895-1-AP (LMNB2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10895-1-AP

  Immunofluorescent analysis of HepG2 cells, using LMNB2 antibody 10895-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of SH-SY5Y using 10895-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using LMNB2 antibody 10895-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

• 

  FC experiment of HEK-293T using 10895-1-AP

  1X10^6 HEK-293T cells were stained with 0.2ug LMNB2 antibody (10895-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, SH-SY5Y cells
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10895-1-AP targets Lamin B2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lamin B2 fusion protein Ag1335
• Full Name: lamin B2
• Calculated molecular weight: 68 kDa
• Observed molecular weight: 66-68 kDa
• GenBank accession number: BC006551
• Gene symbol: LMNB2
• Gene ID (NCBI): 84823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	J Drug Target; Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling.; Authors - Minjuan Wang
human	WB	Aging (Albany NY); MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.; Authors - Zongpu Zhang
human	WB	Bioengineered; Kruppel like factor 16 promotes lung adenocarcinoma progression by upregulating lamin B2.; Authors - Xiaodan Jiao ; KD Validated
human	IHC	IUBMB Life; LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma.; Authors - Weihao Kong
mouse	WB	Chem Biol Interact; HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.; Authors - Xiaoyan Dang
rat	WB	Chem Biol Interact; Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation.; Authors - Yazhi Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Specific bands at predicted size with weak non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000