Lamin B2 Polyclonal antibody

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  WB analysis using 10895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10895-1-AP (LMNB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10895-1-AP

  WB result of Lamin B2 antibody (10895-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B2 transfected HepG2 cells.

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  IP experiment of Jurkat using 10895-1-AP

  IP result of anti-Lamin B2 (IP:10895-1-AP, 3ug; Detection:10895-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human colon cancer using 10895-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10895-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10895-1-AP (Lamin B2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10895-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10895-1-AP (Lamin B2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 10895-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Lamin B2 antibody (10895-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of SH-SY5Y using 10895-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using LMNB2 antibody 10895-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

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  IF Staining of HepG2 using 10895-1-AP

  Immunofluorescent analysis of HepG2 cells, using LMNB2 antibody 10895-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

Lamin B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10895-1-AP

Publications(29)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, Raji cells, mouse brain tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells, SH-SY5Y cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

10895-1-AP targets Lamin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lamin B2 fusion protein Ag1335
• Full Name: lamin B2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 66-68 kDa
• GenBank Accession Number: BC006551
• Gene Symbol: Lamin B2
• Gene ID (NCBI): 84823
• RRID: AB_2136411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q03252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Clin Transl Med; Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.; Authors - Lanqing Wang
human	WB	Aging (Albany NY); MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.; Authors - Zongpu Zhang
mouse	WB	J Drug Target; Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling.; Authors - Minjuan Wang
mouse	WB	Ecotoxicol Environ Saf; Antagonizing effects of curcumin against mercury-induced autophagic death and trace elements disorder by regulating PI3K/AKT and Nrf2 pathway in the spleen.; Authors - Guifang Zhao
human	WB	Front Oncol; Mesenchymal Stem Cells Inhibits Migration and Vasculogenic Mimicry in Nasopharyngeal Carcinoma Via Exosomal MiR-125a.; Authors - Fangzhu Wan
human	WB,IF	Oncogenesis; Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.; Authors - T Kuga ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Specific bands at predicted size with weak non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000