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  • KD/KO Validated

Lamin B2 Polyclonal antibody

Lamin B2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10895-1-AP

Synonyms

LAMB2, lamin B2, LMN2, LMNB2



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, Raji cells, mouse brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, SH-SY5Y cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10895-1-AP targets Lamin B2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lamin B2 fusion protein Ag1335
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 66-68 kDa
GenBank accession numberBC006551
Gene symbol LMNB2
Gene ID (NCBI) 84823
RRIDAB_2136411
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

Protocols

Product Specific Protocols
WB protocol for Lamin B2 antibody 10895-1-APDownload protocol
IHC protocol for Lamin B2 antibody 10895-1-APDownload protocol
IF protocol for Lamin B2 antibody 10895-1-APDownload protocol
IP protocol for Lamin B2 antibody 10895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.

Authors - Lanqing Wang
humanWB

Aging (Albany NY)

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.

Authors - Zongpu Zhang
mouseWB

J Drug Target

Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling.

Authors - Minjuan Wang
mouseWB

Ecotoxicol Environ Saf

Antagonizing effects of curcumin against mercury-induced autophagic death and trace elements disorder by regulating PI3K/AKT and Nrf2 pathway in the spleen.

Authors - Guifang Zhao
humanWB

Front Oncol

Mesenchymal Stem Cells Inhibits Migration and Vasculogenic Mimicry in Nasopharyngeal Carcinoma Via Exosomal MiR-125a.

Authors - Fangzhu Wan
humanWB,IF

Oncogenesis

Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.

Authors - T Kuga
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (03-08-2022)

Specific bands at predicted size with weak non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000