• Featured Product
  • KD/KO Validated

Lamin B2 Polyclonal antibody

Lamin B2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10895-1-AP

Synonyms

LAMB2, lamin B2, LMN2, LMNB2



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, SH-SY5Y cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10895-1-AP targets Lamin B2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lamin B2 fusion protein Ag1335
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 66-68 kDa
GenBank accession numberBC006551
Gene symbol LMNB2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Lamin B2 antibody 10895-1-APDownload protocol
IHC protocol for Lamin B2 antibody 10895-1-APDownload protocol
IF protocol for Lamin B2 antibody 10895-1-APDownload protocol
IP protocol for Lamin B2 antibody 10895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Drug Target

Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling.

Authors - Minjuan Wang
humanWB

Aging (Albany NY)

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.

Authors - Zongpu Zhang
humanWB

Bioengineered

Kruppel like factor 16 promotes lung adenocarcinoma progression by upregulating lamin B2.

Authors - Xiaodan Jiao
  • KD Validated
humanIHC

IUBMB Life

LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma.

Authors - Weihao Kong
mouseWB

Chem Biol Interact

HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.

Authors - Xiaoyan Dang
ratWB

Chem Biol Interact

Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation.

Authors - Yazhi Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (03-08-2022)

Specific bands at predicted size with weak non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000