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Lamin B2 Polyclonal antibody
Lamin B2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10895-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, SH-SY5Y cells |
Positive FC detected in | HEK-293T cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 14 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
10895-1-AP targets Lamin B2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Lamin B2 fusion protein Ag1335 |
Full Name | lamin B2 |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 66-68 kDa |
GenBank accession number | BC006551 |
Gene symbol | LMNB2 |
Gene ID (NCBI) | 84823 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
IHC protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
IF protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
IP protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Drug Target Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling. | ||
Aging (Albany NY) MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma. | ||
Bioengineered Kruppel like factor 16 promotes lung adenocarcinoma progression by upregulating lamin B2.
| ||
IUBMB Life LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma. | ||
Chem Biol Interact HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway. | ||
Chem Biol Interact Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH WEI (Verified Customer) (03-08-2022) | Specific bands at predicted size with weak non-specific bands.
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