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Lamin A/C Polyclonal antibody
Lamin A/C Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10298-1-AP
"Lamin A/C Antibodies" Comparison
View side-by-side comparison of Lamin A/C antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in
|A431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells
|Positive IP detected in
|HeLa cells, A375 cells
|Positive IHC detected in
|mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, HeLa cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:2000-1:8000
|IF : 1:400-1:1600
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10298-1-AP targets Lamin A/C in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, monkey, duck
|Host / Isotype
|Rabbit / IgG
|Lamin A/C fusion protein Ag0408
|Calculated molecular weight
|Observed molecular weight
|65 kDa, 70 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
|Product Specific Protocols
|WB protocol for Lamin A/C antibody 10298-1-AP
|IHC protocol for Lamin A/C antibody 10298-1-AP
|IF protocol for Lamin A/C antibody 10298-1-AP
|IP protocol for Lamin A/C antibody 10298-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Alejandro (Verified Customer) (08-22-2022)
Shows clear staining in IF and well staining using FACS
Charlotte (Verified Customer) (07-29-2022)
Cell fraction performed on NIH-3T3 cells to show the nucleus part. Blot super clean. Antibody specific. Easy to reveal.
S (Verified Customer) (12-31-2021)
Azita (Verified Customer) (06-16-2021)
It was used as a marker in WB to confirm proper nuclear fractionation of cell lysate.
Declan (Verified Customer) (11-29-2018)