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Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10298-1-AP

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin


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  • Western Blot (WB) analysis of various lysates using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis using 10298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis using 10298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis of HeLa using 10298-1-AP

    WB result of Lamin A/C antibody (10298-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.

  • Western Blot (WB) analysis of C6 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis of C6 using 10298-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis of A375 using 10298-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis of HEK-293 using 10298-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis of mouse ovary using 10298-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    WB analysis of SKOV-3 using 10298-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    IP experiment of HeLa using 10298-1-AP

    IP result of anti-Lamin A/C(IP:10298-1-AP, 4ug; Detection:10298-1-AP 1:50000) with HeLa cells lysate 1360 ug.

  • Immunoprecipitation (IP) experiment of A375 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    IP experiment of A375 using 10298-1-AP

    IP Result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Lamin A/C Polyclonal antibody (10298-1-AP)

    IHC staining of mouse heart using 10298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Lamin A/C Polyclonal antibody (10298-1-AP)

    IHC staining of mouse heart using 10298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    IF Staining of HepG2 using 10298-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    IF Staining of HepG2 using 10298-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin A/C antibody (10298-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    IF Staining of HeLa using 10298-1-AP

    Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    IF Staining of HepG2 using 10298-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293T cells using Lamin A/C Polyclonal antibody (10298-1-AP)

    FC experiment of HEK-293T using 10298-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Lamin A/C (10298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

"Lamin A/C Antibodies" Comparison

View side-by-side comparison of Lamin A/C antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells, A375 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10298-1-AP targets Lamin A/C in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lamin A/C fusion protein Ag0408
Full Name lamin A/C
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa, 70 kDa
GenBank accession numberBC003162
Gene symbol LMNA
Gene ID (NCBI) 4000
RRIDAB_2296961
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Protocols

Product Specific Protocols
WB protocol for Lamin A/C antibody 10298-1-APDownload protocol
IHC protocol for Lamin A/C antibody 10298-1-APDownload protocol
IF protocol for Lamin A/C antibody 10298-1-APDownload protocol
IP protocol for Lamin A/C antibody 10298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Microbiol

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.

Authors - Adarsh Dharan
humanWB

Mol Cell

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Authors - Jia Xiong
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Cancer Cell

SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.

Authors - Lan Fang
humanWB

Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alejandro (Verified Customer) (08-22-2022)

Shows clear staining in IF and well staining using FACS

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Bone marrow derived macrophages
Lamin A/C Antibody Immunofluorescence,Flow Cytometry validation (1/100 dilution) in Bone marrow derived macrophages (Cat no:10298-1-AP)
FH

Charlotte (Verified Customer) (07-29-2022)

Cell fraction performed on NIH-3T3 cells to show the nucleus part. Blot super clean. Antibody specific. Easy to reveal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: NIH-3T3
Lamin A/C Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:10298-1-AP)
FH

S (Verified Customer) (12-31-2021)

good antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
Lamin A/C Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:10298-1-AP)
FH

Azita (Verified Customer) (06-16-2021)

It was used as a marker in WB to confirm proper nuclear fractionation of cell lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NSC-34 cell line
FH

Declan (Verified Customer) (11-29-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: Human brain homogenate