Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HepG2 cells, A431 cells, HeLa cells, MCF-7 cells, COLO 320 cells, Jurkat cells
|Positive IP detected in
|mouse skeletal muscle tissue
|Positive IHC detected in
|human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10351-1-AP targets Emerin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|Emerin fusion protein Ag0236
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
Cytoplasmic control of intranuclear polarity by human cytomegalovirus.
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.
Off-the-shelf proximity biotinylation for interaction proteomics.
Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.
J Cell Biol
Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-12-2019)
Antibody recognizes clear band at the corrected expected molecular weight of Emerin.