Emerin Polyclonal antibody

Emerin Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 10351-1-AP

Synonyms

EDMD, EMD, Emerin, LEMD5, STA

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Validation Data Gallery

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  WB analysis using 10351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10351-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10351-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10351-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10351-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10351-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10351-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10351-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle tissue using 10351-1-AP

  IP result of EMD antibody (10351-1-AP for IP and Detection) with mouse skeletal muscle tissue lysate.

• 

  IF Staining of HeLa cells using 10351-1-AP

  IF result of anti-EMD (10351-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

• 

  IF Staining of HeLa using 10351-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (10351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 10351-1-AP

  1X10^6 HEK-293T cells were stained with 0.2ug Emerin antibody (10351-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, A431 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IF detected in: HeLa cells
• Positive FC detected in: HEK-293T cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 12 publications below
• IP: See 1 publications below

Product Information

10351-1-AP targets Emerin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Emerin fusion protein Ag0236
• Full Name: emerin
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC000738
• Gene symbol: EMD
• Gene ID (NCBI): 2010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane.; Authors - Marina Blenski
human	WB,IF	Cells; Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.; Authors - Magda Dubińska-Magiera
mouse	WB	Cells; EDMD-Causing Emerin Mutant Myogenic Progenitors Exhibit Impaired Differentiation Using Similar Mechanisms.; Authors - Ashvin Iyer
human	WB	Cell Rep; Recruitment of DNA to tumor-derived microvesicles.; Authors - James W Clancy
human	WB,IHC	World J Surg Oncol; Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.; Authors - Tianzhen He
mouse	WB	BMC Cell Biol; Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.; Authors - Elizabeth R Smith

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-12-2019)

Antibody recognizes clear band at the corrected expected molecular weight of Emerin.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Emerin Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10351-1-AP)

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