Emerin Polyclonal antibody

Emerin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10351-1-AP

Synonyms

EDMD, EMD, Emerin, LEMD5, STA



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, A431 cells, HeLa cells, MCF-7 cells, COLO 320 cells, Jurkat cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10351-1-AP targets Emerin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Emerin fusion protein Ag0236
Full Name emerin
Calculated molecular weight 34 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
RRIDAB_2100056
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for Emerin antibody 10351-1-APDownload protocol
IHC protocol for Emerin antibody 10351-1-APDownload protocol
IF protocol for Emerin antibody 10351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Authors - Dean J Procter
humanIF

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
IF

Cell

DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.

Authors - Matthias Samwer
humanIF,IP

Nat Commun

Off-the-shelf proximity biotinylation for interaction proteomics.

Authors - Irene Santos-Barriopedro
humanIF

Dev Cell

Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

Authors - Holly Merta
humanIF

J Cell Biol

Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.

Authors - Banafsheh Golchoubian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-12-2019)

Antibody recognizes clear band at the corrected expected molecular weight of Emerin.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Emerin Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10351-1-AP)