Emerin Polyclonal antibody
Emerin Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10351-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, A431 cells |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IF detected in | HeLa cells |
Positive FC detected in | HEK-293T cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 12 publications below |
IHC | See 1 publications below |
IF | See 12 publications below |
IP | See 1 publications below |
Product Information
10351-1-AP targets Emerin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Emerin fusion protein Ag0236 |
Full Name | emerin |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 35 kDa |
GenBank accession number | BC000738 |
Gene symbol | EMD |
Gene ID (NCBI) | 2010 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Emerin antibody 10351-1-AP | Download protocol |
IF protocol for Emerin antibody 10351-1-AP | Download protocol |
FC protocol for Emerin antibody 10351-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane. | ||
Cells Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients. | ||
Cells EDMD-Causing Emerin Mutant Myogenic Progenitors Exhibit Impaired Differentiation Using Similar Mechanisms. | ||
World J Surg Oncol Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer. | ||
BMC Cell Biol Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joleen (Verified Customer) (06-12-2019) | Antibody recognizes clear band at the corrected expected molecular weight of Emerin.
![]() |