Emerin Polyclonal antibody

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  WB analysis using 10351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10351-1-AP

  WB result of Emerin antibody (10351-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells.

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  WB analysis of MCF-7 using 10351-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10351-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10351-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10351-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10351-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10351-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10351-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10351-1-AP (Emerin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle tissue using 10351-1-AP

  IP result of EMD antibody (10351-1-AP for IP and Detection) with mouse skeletal muscle tissue lysate.

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  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 10351-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10351-1-AP (Emerin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa cells using 10351-1-AP

  IF result of anti-EMD (10351-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

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  IF Staining of HeLa using 10351-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (10351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 10351-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Emerin (10351-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Emerin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10351-1-AP

Publications(32)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

EMD, STA, LEMD5, EDMD

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 18 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

10351-1-AP targets Emerin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Emerin fusion protein Ag0236
• Full Name: emerin
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000738
• Gene Symbol: EMD
• Gene ID (NCBI): 2010
• RRID: AB_2100056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50402
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Publications

Species	Application	Title
human	WB,IF	Nature; Cytoplasmic control of intranuclear polarity by human cytomegalovirus.; Authors - Dean J Procter
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
	IF	Cell; DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.; Authors - Matthias Samwer
human	IF,IP	Nat Commun; Off-the-shelf proximity biotinylation for interaction proteomics.; Authors - Irene Santos-Barriopedro
human	IF	Dev Cell; Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.; Authors - Holly Merta
human	IF	J Cell Biol; Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.; Authors - Banafsheh Golchoubian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-12-2019)

Antibody recognizes clear band at the corrected expected molecular weight of Emerin.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Emerin Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10351-1-AP)