Anticorps Polyclonal de lapin anti-HuR/ELAVL1

• Phare
• Validé par KD/KO

HuR/ELAVL1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, CLIP

Conjugaison

Non conjugué

Publications

82

Avis (6)

N° de cat : 11910-1-AP

Synonymes

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

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Galerie de données de validation

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  WB analysis using 11910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR/ELAVL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11910-1-AP

  WB result of HuR/ELAVL1 antibody (11910-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 11910-1-AP

  IP result of anti-HuR/ELAVL1 (IP:11910-1-AP, 4ug; Detection:11910-1-AP 1:20000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human breast cancer using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11910-1-AP (HuR/ELAVL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11910-1-AP (HuR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11910-1-AP (HuR/ELAVL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11910-1-AP (HuR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HuR/ELAVL1 antibody (11910-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 11910-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human HuR/ELAVL1 (11910-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 59 publications below
• IHC: See 9 publications below
• IF: See 20 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 7 publications below

Informations sur le produit

11910-1-AP cible HuR/ELAVL1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, CLIP et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HuR/ELAVL1 Protéine recombinante Ag2249
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 30-37 kDa
• Numéro d’acquisition GenBank: BC003376
• Symbole du gène: HuR/ELAVL1
• Identification du gène (NCBI): 1994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Publications

Species	Application	Title
human	WB	Nat Cancer; SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers; Authors - Lei Shi
human	WB	Nat Protoc; Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.; Authors - Joel I Perez-Perri
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Autophagy; m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin; Authors - Diefei Liang ; KD Validated
human	WB	Nat Commun; Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture.; Authors - Joel I Perez-Perri

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Adrianna (Verified Customer) (10-24-2022)

This antibody works very well - strong detection without unspecific bands. I usually incubate it over night at 4 deg C.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

FH

PK (Verified Customer) (07-08-2022)

Very Good

• Applications: Western Blot
• Cell Tissue Type: H9C2

HuR/ELAVL1 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:11910-1-AP)

FH

S (Verified Customer) (04-19-2021)

Excellent!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

HuR/ELAVL1 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)

FH

S (Verified Customer) (05-18-2020)

Good Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

HuR/ELAVL1 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)

FH

CHAO (Verified Customer) (01-30-2020)

Weak band for the heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Gaston (Verified Customer) (05-16-2019)

Antibody worked very well for wester blot detection and immunofluorescence in Huh7 and SH-SY5Y cells. Did not observe any signs of deteriorating detection when stored at -20 for over two years.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:250; 1:6500
• Cell Tissue Type: Huh7, SH-SY5Y, Vero

HuR/ELAVL1 Antibody Western Blot,Immunofluorescence, validation (1:250; 1:6500 dilution) in Huh7, SH-SY5Y, Vero (Cat no:11910-1-AP)

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