HNRNPD Polyclonal antibody

• Featured Product
• KD/KO Validated

HNRNPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 12770-1-AP

Synonyms

AUF1, HNRPD, hnRNPD0, hnRNP D0, AU-rich element RNA-binding protein 1

---

Validation Data Gallery

• 

  WB analysis using 12770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12770-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12770-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12770-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12770-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12770-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 12770-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (12770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue, human normal colon, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

12770-1-AP targets HNRNPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPD fusion protein Ag3458
• Full Name: heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
• Calculated Molecular Weight: 355 aa, 38 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC026015
• Gene Symbol: HNRNPD
• Gene ID (NCBI): 3184
• RRID: AB_2117318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

Publications

Species	Application	Title
human	WB	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	IHC	Brain; Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions.; Authors - Johnathan Cooper-Knock
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	EMBO Rep; Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates.; Authors - Johan Aarum