Anticorps Polyclonal de lapin anti-HNF1B

• Phare
• Validé par KD/KO

HNF1B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 12533-1-AP

Synonymes

FJHN, HNF 1 beta, HNF 1B, HNF1 homeobox B, HNF1B, HNF1beta, HNF2, Homeoprotein LFB3, HPC11, LF B3, LFB3, MODY5, TCF 2, TCF2, Transcription factor 2, VHNF1

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Galerie de données de validation

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  WB analysis of L02 using 12533-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 12533-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12533-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 12533-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules Jurkat, cellules MDCK
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 11 publications below
• IF: See 3 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12533-1-AP cible HNF1B dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNF1B Protéine recombinante Ag3232
• Nom complet: HNF1 homeobox B
• Masse moléculaire calculée: 557 aa, 61 kDa
• Poids moléculaire observé: 58-60 kDa
• Numéro d’acquisition GenBank: BC017714
• Symbole du gène: HNF1B
• Identification du gène (NCBI): 6928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.

Publications

Species	Application	Title
human	IHC	Elife; Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.; Authors - Marko Z Nikolić
human	WB,IHC	Oncogene; HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.; Authors - Jianqing Wang ; KD Validated
mouse	IF	Development; Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment.; Authors - Asha Seth
pig	IF	Sci Rep; Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.; Authors - Phillip L Lewis
human	WB,IHC	J Cell Mol Med; HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.; Authors - Wei Lu
mouse	IHC	Sci Rep; ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.; Authors - Yiannis Drosos

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