KIF12 Polyclonal antibody

KIF12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Publications

5

Cat no : 12035-1-AP

Synonyms

RP11 56P10.3, Kinesin-like protein KIF12, Kinesin like protein KIF12

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Validation Data Gallery

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  WB analysis using 12035-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12035-1-AP (KIF12 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12035-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12035-1-AP (KIF12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12035-1-AP (KIF12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, Y79 cells, mouse pancreas tissue, rat kidney tissue, mouse heart tissue
• Positive IHC detected in: human kidney tissue, human pancreas cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

12035-1-AP targets KIF12 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF12 fusion protein Ag2707
• Full Name: kinesin family member 12
• Calculated Molecular Weight: 646 aa, 71 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC010626
• Gene Symbol: KIF12
• Gene ID (NCBI): 113220
• RRID: AB_2280813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; HNF-1beta regulates transcription of the PKD modifier gene Kif12.; Authors - Gong Yimei Y
mouse, human	IF	Kidney Int; Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.; Authors - Rik Westland
human	WB	Breast Cancer Res Treat; Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer.; Authors - Tan Min Han MH
human	IHC	J Proteomics; Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.; Authors - Prunotto Marco M
human	WB,IF	J Pediatr; KIF12 Variants and Disturbed Hepatocyte Polarity in Children With a Phenotypic Spectrum of Cholestatic Liver Disease.; Authors - Stalke Amelie