TMEM27 Polyclonal antibody

TMEM27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19207-1-AP

Synonyms

ACE2, CLTRN, Collectrin, NX 17, NX17, TMEM27, transmembrane protein 27

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Validation Data Gallery

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  WB analysis of mouse pancreas using 19207-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19207-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19207-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 19207-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19207-1-AP (TMEM27 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 19207-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19207-1-AP (TMEM27 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 19207-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19207-1-AP (TMEM27 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19207-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19207-1-AP (TMEM27 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19207-1-AP

  Immunofluorescent analysis of HepG2 cells, using TMEM27 antibody 19207-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19207-1-AP targets TMEM27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM27 fusion protein Ag7668
• Full Name: transmembrane protein 27
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC050606
• Gene Symbol: TMEM27
• Gene ID (NCBI): 57393
• RRID: AB_10860104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated ins secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).