Uromodulin Polyclonal antibody

Uromodulin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11911-1-AP

Synonyms

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin



Tested Applications

Positive WB detected inhuman urine sample, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11911-1-AP targets Uromodulin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Uromodulin fusion protein Ag2494
Full Name uromodulin
Calculated Molecular Weight 640 aa, 70 kDa
Observed Molecular Weight90-105 kDa, 55-75 kDa
GenBank Accession NumberBC035975
Gene Symbol Uromodulin
Gene ID (NCBI) 7369
RRIDAB_10695625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

Protocols

Product Specific Protocols
WB protocol for Uromodulin antibody 11911-1-APDownload protocol
IHC protocol for Uromodulin antibody 11911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Sci China Life Sci

Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples.

Authors - An-Jing Chen
humanIHC

Kidney Int Rep

Vancomycin-Associated Tubular Casts and Vancomycin Nephrotoxicity.

Authors - Ngoentra Tantranont
humanIHC

Nephrology (Carlton)

Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy.

Authors - Keiko Tanaka