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Cadherin-16 Polyclonal antibody

Cadherin-16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15107-1-AP

Synonyms

cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin



Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, HEK-293 cells, rat kidney tissue
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15107-1-AP targets Cadherin-16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cadherin-16 fusion protein Ag4343
Full Name cadherin 16, KSP-cadherin
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC027912
Gene Symbol Cadherin-16
Gene ID (NCBI) 1014
RRIDAB_10596931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

Protocols

Product Specific Protocols
WB protocol for Cadherin-16 antibody 15107-1-APDownload protocol
IHC protocol for Cadherin-16 antibody 15107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Int Immunopharmacol

Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion

Authors - Zhi Zhao
mouseWB,IF

Front Cell Dev Biol

Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition.

Authors - Yanhong Zhang
mouseWB,IF

J Cell Sci

Pax8 controls thyroid follicular polarity through Cadherin-16.

Authors - Petrina Koumarianou
  • KD Validated
humanWB

J Cancer

TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers.

Authors - Jiaqi Dai
humanWB

Oncol Lett

Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

Authors - Xiaolin Yang
mouseIF

Cell Death Dis

Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis.

Authors - Huzi Xu