PCBD1 Polyclonal antibody

PCBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15702-1-AP

Synonyms

PCD, PCBD, EC:4.2.1.96, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCOH

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Validation Data Gallery

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  WB analysis using 15702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15702-1-AP

  Immunofluorescent analysis of Hela cells, using PCBD1 antibody 15702-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15702-1-AP targets PCBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCBD1 fusion protein Ag8331
• Full Name: pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
• Calculated Molecular Weight: 104 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC006324
• Gene Symbol: PCBD1
• Gene ID (NCBI): 5092
• RRID: AB_2267945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCBD1, also known as DCOH and PCBD, belongs to the pterin-4-alpha-carbinolamine dehydratase family. PCBD1 is a bifunctional protein that acts as an enzyme in the regeneration of cofactor tetrahydrobiopterin (BH4), crucial for the function of aromatic amino acid hydroxylases, and as a dimerization cofactor of transcription factors HNF1A and HNF1B, important in liver and pancreas development and function. PCBD1 is expressed in the developing pancreas. The calculated molecular weight of PCBD1 is 12 kDa (PMID: 24204001, 24848070).