Anticorps Polyclonal de lapin anti-HINT1

• Phare
• Validé par KD/KO

HINT1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 10717-1-AP

Synonymes

HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

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Galerie de données de validation

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  WB analysis using 10717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 10717-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10717-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10717-1-AP

  IP result of anti-HINT1 (IP:10717-1-AP, 3ug; Detection:10717-1-AP 1:500) with mouse brain tissue lysate 7500ug.

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  IHC staining of human colon cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules Raji, tissu cérébral de rat, tissu de thymus de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

10717-1-AP cible HINT1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HINT1 Protéine recombinante Ag1142
• Nom complet: histidine triad nucleotide binding protein 1
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC007090
• Symbole du gène: HINT1
• Identification du gène (NCBI): 3094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.

Publications

Species	Application	Title
human	IF	Nat Commun; Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.; Authors - Jing Yu
		Oncogene; Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.; Authors - A Motzik
human	WB	J Med Chem; A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide; Authors - Kellan T. Passow
human	IHC	Cell Cycle; The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.; Authors - Genovese Giannicola G
human,mouse	WB	Pharmacogenomics J; Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.; Authors - Jackson K J KJ
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J

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