FHIT Monoclonal antibody

FHIT Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4D6G1

Cat no : 66086-1-Ig

Synonyms

AP3A hydrolase, AP3Aase, Bis(5 adenosyl) triphosphatase, Dinucleosidetriphosphatase, FHIT, FRA3B, fragile histidine triad gene

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Validation Data Gallery

• 

  WB analysis of K-562 using 66086-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66086-1-Ig (FHIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 66086-1-Ig is FHIT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FHIT fusion protein Ag14471
• Full Name: fragile histidine triad gene
• Calculated Molecular Weight: 147 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC032336
• Gene Symbol: FHIT
• Gene ID (NCBI): 2272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.

Publications

Species	Application	Title
human	IHC	Cancer Biomark; FHIT and C-MYC expression in cervical histology and cytology as biomarkers for detecting high-grade intraepithelial neoplasia in human papillomavirus-positive women.; Authors - Fan Yang
human	IHC,WB	Int J Clin Exp Pathol; Upregulation of FHIT gene expression in endometrial carcinoma by RNA activation.; Authors - Qing Zhu