GDI2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GDI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 10116-1-AP

Synonyms

RABGDIB, Rab GDI β, Rab GDI beta, GDP dissociation inhibitor 2, GDI-2

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Validation Data Gallery

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  WB analysis of A431 using 10116-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10116-1-AP (GDI2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10116-1-AP (GDI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, rat brain tissue, mouse spleen tissue, rat spleen tissue, HeLa cells, mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human colon tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

10116-1-AP targets GDI2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDI2 fusion protein Ag0170
• Full Name: GDP dissociation inhibitor 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC005145
• Gene Symbol: GDI2
• Gene ID (NCBI): 2665
• RRID: AB_2279073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

Publications

Species	Application	Title
monkey	WB	Science; A bifunctional bacterial protein links GDI displacement to Rab1 activation.; Authors - Machner Matthias P MP
human	WB	Nat Chem Biol; Enhanced mapping of small-molecule binding sites in cells; Authors - Jacob M Wozniak
yeast	WB	Proc Natl Acad Sci U S A; Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA.; Authors - Zhu Yongqun Y
mouse	IF	Curr Biol; Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.; Authors - Anil Kumar Ganga
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
mouse	WB	Mol Cell Proteomics; Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene.; Authors - Wishart Thomas M TM

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guo-rong (Verified Customer) (08-30-2022)

Specific and clear results

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ARPE-19

GDI2 Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:10116-1-AP)