Beta Arrestin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Beta Arrestin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 10171-1-AP

Synonyms

ARRB2, β-Arrestin 2, Beta-arrestin-2, BARR2, Arrestin beta-2

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Validation Data Gallery

• 

  WB analysis using 10171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 10171-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10171-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10171-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10171-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsil using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsil using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10171-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Arrestin 2 antibody (10171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of mouse Retina tissue using 10171-1-AP

  IF result of anti-ARRB2 (10171-1-AP) in Mouse Retina by Dr. Seongjin Seo. IS (Inner segment) Stain.

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Tested Applications

• Positive WB detected in: mouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue
• Positive IHC detected in: human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, mouse Retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

10171-1-AP targets Beta Arrestin 2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Arrestin 2 fusion protein Ag0227
• Full Name: arrestin, beta 2
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC007427
• Gene Symbol: Beta Arrestin 2
• Gene ID (NCBI): 409
• RRID: AB_10644158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.; Authors - Yuqing Wu ; KO Validated
mouse	WB	Sci Adv; Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome; Authors - Wei Wang
rat	WB	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
human,mouse	WB,IF,CoIP	J Pharm Anal; Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis; Authors - Jia-Jia Zhang ; KD Validated
mouse	IF	J Cell Biol; BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.; Authors - Fan Ye
mouse	IF	Acta Pharmacol Sin; Activation of GPR120 in podocytes ameliorates kidney fibrosis and inflammation in diabetic nephropathy.; Authors - Tian-Tian Wei

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