Anticorps Polyclonal de lapin anti-GFAP

• Phare
• Validé par KD/KO

GFAP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications

505

Avis (8)

N° de cat : 16825-1-AP

Synonymes

glial fibrillary acidic protein

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Galerie de données de validation

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  WB analysis of U-251 using 16825-1-AP

  WB result of GFAP antibody (16825-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells.

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  WB analysis using 16825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16825-1-AP (GFAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of Alzheimer mouse using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded Hippocampus tissue slide from Alzheimer Mouse Model using 16825-1-AP (GFAP antibody) at dilution of 1:50000 (under 10x lens). Data from NeuroScience Associates, Inc.

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  IHC staining of mouse brain using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 16825-1-AP (GFAP antibody) at dilution of 1:1000 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules U-251, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, souris Alzheimer; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF-Fro: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 148 publications below
• IHC: See 83 publications below
• IF: See 340 publications below

Informations sur le produit

16825-1-AP cible GFAP dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, souris, Hamster, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFAP Protéine recombinante Ag10423
• Nom complet: glial fibrillary acidic protein
• Masse moléculaire calculée: 432 aa, 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC013596
• Symbole du gène: GFAP
• Identification du gène (NCBI): 2670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Publications

Species	Application	Title
mouse	IF	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
mouse	IF	Nat Metab; Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice; Authors - Wan-Ting Dong
human	IHC	Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue
	WB	Nat Commun; Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury; Authors - Airi Jo-Watanabe
rat	IF	J Extracell Vesicles; Neuron-derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB-mediated signalling and preserve neuronal complexity; Authors - Julia Solana-Balaguer
mouse	WB,IF	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vikas (Verified Customer) (07-11-2024)

Used for IF, Worked very well, Highly recommended.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Brain tissue

FH

manohar (Verified Customer) (03-06-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Badrieh (Verified Customer) (08-02-2022)

this Antibody worked really great with different Recombinant GFAP proteins in ELISA.

• Applications: Other

FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence works well on U251-MG human astrocytoma cell line

• Applications: Immunohistochemistry, Immunofluorescence

FH

Azita (Verified Customer) (05-31-2021)

The human primary cortical cells (DIV28) were subjected to ICC using GFAP antibody (at 1/500 dilution) overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human primary cortical cells

FH

Diane (Verified Customer) (01-03-2020)

I have used this antibody successfully for formalin-fixed paraffin-embedded brain tissues in humans, mouse and rat. I am impressed that there is no non-specific background staining regardless of species. We have used the antibody in a double-staining technique and were able to achieve crisp diagnostic staining for images. The astrocytes were stained using alkaline phosphatase Permanent Red. Antigen retrieval was performed using proteinase K.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: gliotic brain tissue - human

GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in gliotic brain tissue - human (Cat no:16825-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good immuno-fluorescence and western blotting analysis using mouse brain tissues.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph=6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Zebrafish retina 5dpf

GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in Zebrafish retina 5dpf (Cat no:16825-1-AP)

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