S100 Beta Polyclonal antibody
S100 Beta Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15146-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, human brain tissue, human testis tissue, mouse brain tissue, U-251 cells, C6 cells |
| Positive IHC detected in | human malignant melanoma tissue, human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue, A375 cells, human astrocytes, human malignant melanoma tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 10 publications below |
| IHC | See 13 publications below |
| IF | See 36 publications below |
| FC | See 1 publications below |
Product Information
15146-1-AP targets S100 Beta in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | S100 Beta fusion protein Ag7440 |
| Full Name | S100 calcium binding protein B |
| Calculated molecular weight | 11 kDa |
| Observed molecular weight | 11 kDa |
| GenBank accession number | BC001766 |
| Gene symbol | S100B |
| Gene ID (NCBI) | 6285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
S100B belongs to the EF-band calcium binding proteins and is found primarily in astrocytes in the central nervous system (CNS). S100B has a variety of functions, including calcium homeostasis, cell proliferation, differentiation, migration, and survival, as well as neurite outgrowth and regeneration.
1. What is the molecular weight of S100B?
S100B protein is composed of non-covalently linked homodimers of 11 kDa size.
2. What is the subcellular localization of S100B?
S100B localizes to the nucleus and cytoplasm, associating with intracellular membranes, the centrosomes, microtubules, and type III intermediate filaments (PMID: 19110011). Additionally, it can be released from astrocytes into the extracellular space and can enter the bloodstream.
3. What is the expression pattern of S100B?
S100B is predominantly expressed in astrocytes and maturing oligodendrocytes but is also present in other cell types such as kidney epithelial cells, neural progenitor cells, pituicytes, ependymocytes, chondrocytes, adipocytes, melanocytes, Langerhans cells, dendritic cells, certain lymphocyte subpopulations, skeletal myofibers, myoblasts, and muscle satellite cells (PMID: 19110011). S100B is a commonly used marker of Schwann cells and reactive astrocytes in ICC, IHC, and WB applications.
4. What is the diagnostic use of S100B in the clinic?
S100B is naturally secreted by astrocytes into the extracellular space and low amounts of S100B can pass through the brain-blood barrier and enter the bloodstream. Elevated levels of S100B in the serum are observed in patients with traumatic head injuries, as well as in patients suffering from neurodegenerative diseases (PMID: 30144068). This increase in S100B levels is attributed to the elevated secretion of S100B protein from astrocytes as part of the physiological response to the injury, as well as to the physical damage of astrocytes and increased blood-brain barrier permeability.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S100 Beta antibody 15146-1-AP | Download protocol |
| IHC protocol for S100 Beta antibody 15146-1-AP | Download protocol |
| IF protocol for S100 Beta antibody 15146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mechanoreceptor synapses in the brainstem shape the central representation of touch. | ||
Small Methods Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration | ||
J Pineal Res The protective effect of melatonin on brain ischemia and reperfusion in rats and humans: in vivo assessment and a randomized controlled trial. | ||
Nucleic Acids Res H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Clarisse (Verified Customer) (08-08-2022) | This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.
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FH Tongcheng (Verified Customer) (09-08-2021) | This antibody works perfect on human astrocytes.
|
FH Sonia (Verified Customer) (10-21-2019) | Works really well on mouse sciatic nerve cross section (cryostat)
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