GFAP Monoclonal antibody

GFAP Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IP, IHC, IF, Dot blot, ELISA

Conjugate

Unconjugated

CloneNo.

4B2E10

Cat no : 60190-1-Ig

Synonyms

GFAP



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, pig brain tissue, U-251 cells, rat cerebellum, mouse brain, mouse cerebellum, rabbit brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Product Information

60190-1-Ig targets GFAP in WB, IP, IHC, IF, Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFAP fusion protein Ag10452
Full Name glial fibrillary acidic protein
Calculated molecular weight 432 aa, 50 kDa
Observed molecular weight 45-52 kDa
GenBank accession numberBC013596
Gene symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.
Tissue specificity
GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease
  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.
Isoforms
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.



Protocols

Product Specific Protocols
WB protocol for GFAP antibody 60190-1-IgDownload protocol
IHC protocol for GFAP antibody 60190-1-IgDownload protocol
IF protocol for GFAP antibody 60190-1-IgDownload protocol
IP protocol for GFAP antibody 60190-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
mouseWB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
mouseIF

Sci Adv

GPR40 modulates epileptic seizure and NMDA receptor function.

Authors - Yong Yang
ratIF

Small

A Multichannel Flexible Optoelectronic Fiber Device for Distributed Implantable Neurological Stimulation and Monitoring.

Authors - Jingxian Yu
mouseIF

Autophagy

Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.

Authors - M Iqbal Hossain
mouseWB,IF

Mol Neurodegener

Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease.

Authors - Guoxin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

B (Verified Customer) (01-05-2022)

Very clear and strong signal in WB with a right MW about 50 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
GFAP Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:60190-1-Ig)
FH

Mai (Verified Customer) (01-30-2020)

The antibody seemed to have some background. I will repeat the experiment with different dilutions and conditions to find an optimum dilution for this antibody.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Brain Tissue
FH

George (Verified Customer) (08-27-2019)

This gave good staining for activated astrocytes in the 488 channel (1:500 secondary dilution) however there was also significant endogenous background staining which made analysis difficult.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Spinal Cord Mouse Slice Culture
FH

Chintan (Verified Customer) (06-03-2019)

This antibody was used to detect Glial fibrillary acidic protein in primary mouse microglia cells using Western blot. Antibody worked quite well and gave a very specific band near 50 kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: Mouse microglia
GFAP Antibody Western Blot, validation (1:10,000 dilution) in Mouse microglia (Cat no:60190-1-Ig)
FH

Di (Verified Customer) (12-18-2018)

Good antibody!

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Neurons
FH

Lalitha (Verified Customer) (12-13-2018)

Reliable results.

  • Applications: Western Blot, Immunohistochemistry,
  • Cell Tissue Type: Mice Brain
FH

Chandrakanth (Verified Customer) (12-06-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cerebellum
FH

Emily (Verified Customer) (11-29-2018)

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse brain sections, mouse whole retina, mouse optic nerve sections