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Anticorps Monoclonal anti-Fibrinogen Gamma Chain

Fibrinogen Gamma Chain Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain

Applications

WB, IHC, IF

Conjugaison

Non conjugué

CloneNo.

2B2B8

N° de cat : 66158-1-Ig

Synonymes

FGG, fibrinogen gamma chain

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of L02 cells using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    WB analysis of L02 using 66158-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human liver using 66158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human liver using 66158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human kidney using 66158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human kidney using 66158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IF Staining of L02 using 66158-1-Ig

    Immunofluorescent analysis of L02 cells using 66158-1-Ig (Fibrinogen gamma chain antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Applications testées

Résultats positifs en WBcellules L02
Résultats positifs en IHCtissu hépatique humain, tissu rénal humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules L02

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

IHCSee 1 publications below

Informations sur le produit

66158-1-Ig cible Fibrinogen Gamma Chain dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Mouse / IgM
Clonalité Monoclonal
Type Anticorps
Immunogène Fibrinogen Gamma Chain Protéine recombinante Ag8817
Nom complet fibrinogen gamma chain
Masse moléculaire calculée453aa,52 kDa; 437aa,50 kDa
Poids moléculaire observé 52 kDa
Numéro d’acquisition GenBankBC007044
Symbole du gène FGG
Identification du gène (NCBI) 2266
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Chromatographie d'affinité thiophilique
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Protocole

Product Specific Protocols
WB protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
IHC protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
IF protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Elevated Serum FGG Levels Prognosticate and Promote the Disease Progression in Prostate Cancer.

Authors - H H Peng
View Article

Mol Ther Nucleic Acids

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.

Authors - Fanfan Li
View Article