Fibrinogen Gamma Chain Polyclonal antibody

• Featured Product
• KD/KO Validated

Fibrinogen Gamma Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 15841-1-AP

Synonyms

FGG, PRO2061

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Validation Data Gallery

• 

  WB analysis of human spleen using 15841-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen Gamma Chain antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15841-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15841-1-AP (Fibrinogen Gamma Chain antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, human spleen tissue, mouse liver tissue, mouse spleen tissue, rat spleen tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Product Information

15841-1-AP targets Fibrinogen Gamma Chain in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibrinogen Gamma Chain fusion protein Ag8597
• Full Name: fibrinogen gamma chain
• Calculated Molecular Weight: 453aa,52 kDa; 437aa,50 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007044
• Gene Symbol: Fibrinogen Gamma Chain
• Gene ID (NCBI): 2266
• RRID: AB_2103801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Publications

Species	Application	Title
human,rat	IHC,IF	Blood; Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.; Authors - Krishnamoorthy Aparna A
mouse		J Thromb Haemost; Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.; Authors - L G Poole
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
rat	IHC	J Neurotrauma; FTY720 Attenuates Neuropathic Pain after Spinal Cord Injury by Decreasing Systemic and Local Inflammation in a Rat Spinal Cord Compression Model.; Authors - Kazuyoshi Yamazaki
human	WB,IHC,IF	Front Neurosci; Fibrinogen Chains Intrinsic to the Brain.; Authors - Eugene V Golanov ; KD Validated
mouse	IHC	Cell Mol Neurobiol; Myelin Debris Impairs Tight Junctions and Promotes the Migration of Microvascular Endothelial Cells in the Injured Spinal Cord.; Authors - Fei Yao

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