F2 Polyclonal antibody

F2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 24295-1-AP

Synonyms

Coagulation factor II, PT, Prothrombin, EC:3.4.21.5, Activation peptide fragment 2

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Validation Data Gallery

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  WB analysis of human plasma using 24295-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24295-1-AP (F2 Antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 24295-1-AP

  IP result of anti-F2 (IP:24295-1-AP, 4ug; Detection:24295-1-AP 1:10000) with human plasma lysate 4000ug.

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  IF Staining of HUVEC using 24295-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 24295-1-AP (F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IP detected in: human plasma tissue
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below

Product Information

24295-1-AP targets F2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, plasmodium falciparum
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F2 fusion protein Ag18385
• Full Name: coagulation factor II (prothrombin)
• Calculated Molecular Weight: 622 aa, 70 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC051332
• Gene Symbol: F2
• Gene ID (NCBI): 2147
• RRID: AB_2879478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Sci Rep; Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.; Authors - Takahiro Tougan
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
Plasmodium falciparum	WB,IF	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
human	IF	PLoS Pathog; Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication; Authors - Danielle M Pierce

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elke (Verified Customer) (02-23-2023)

it can work on the western blot

• Applications: Western Blot