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Fibrinogen Beta Chain Monoclonal antibody

Fibrinogen Beta Chain Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F8E4

Cat no : 66186-1-Ig

Synonyms

FGB, fibrinogen beta chain

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using Fibrinogen Beta Chain Monoclonal antibody (66186-1-Ig)

    WB analysis of human heart using 66186-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66186-1-Ig (Fibrinogen beta chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using Fibrinogen Beta Chain Monoclonal antibody (66186-1-Ig)

    WB analysis of fetal human brain using 66186-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66186-1-Ig (Fibrinogen beta chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman heart tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66186-1-Ig targets Fibrinogen Beta Chain in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Fibrinogen Beta Chain fusion protein Ag10205
Full Name fibrinogen beta chain
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC107766
Gene Symbol Fibrinogen Beta Chain
Gene ID (NCBI) 2244
RRIDAB_2881581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

Protocols

Product Specific Protocols
WB protocol for Fibrinogen Beta Chain antibody 66186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann Transl Med

LncRNA ABCC6P1 promotes proliferation and migration of papillary thyroid cancer cells via Wnt/β-catenin signaling pathway.

Authors - Yue Guan
View Article
human

Int J Surg

Identification and validation of the surface proteins FIBG, PDGF-β, and TGF-β on serum extracellular vesicles for non-invasive detection of colorectal cancer: experimental study

Authors - Zhijian Huang
View Article