Anticorps Polyclonal de lapin anti-FAF1

• Phare
• Validé par KD/KO

FAF1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10271-1-AP

Synonymes

CGI 03, FAF1, FAS associated factor 1, hFAF1, HFAF1s, UBXD12, UBXN3A

---

Galerie de données de validation

• 

  WB analysis of HeLa using 10271-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10271-1-AP

  WB result of FAF1 antibody (10271-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF1 transfected HEK-293 cells.

• 

  WB analysis of mouse testis using 10271-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10271-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10271-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10271-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10271-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10271-1-AP (FAF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10271-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10271-1-AP (FAF1 antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules K-562, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

10271-1-AP cible FAF1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAF1 Protéine recombinante Ag0407
• Nom complet: Fas (TNFRSF6) associated factor 1
• Masse moléculaire calculée: 650aa, 74 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC004970
• Symbole du gène: FAF1
• Identification du gène (NCBI): 11124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.

Publications

Species	Application	Title
human	WB,IF	Cell Death Differ; FAF1 mediates regulated necrosis through PARP1 activation upon oxidative stress leading to dopaminergic neurodegeneration.; Authors - Changsun Yu
human, mouse	WB,IHC,IF	Hum Mol Genet; Accumulation of the parkin substrate, FAF1, plays a key role in the dopaminergic neurodegeneration.; Authors - Sul Jee-Won JW
mouse	WB,IHC,IF	J Neurosci; Fas-associated factor 1 as a regulator of olfactory axon guidance.; Authors - Cheng Kai K
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
human	WB	Cancer Manag Res; MiR-26a-5p Serves as an Oncogenic MicroRNA in Non-Small Cell Lung Cancer by Targeting FAF1.; Authors - Ming-Fan Ye ; KD Validated
mouse	IF	Neuroreport; Fas-associated factor 1 promotes in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice.; Authors - Jong Mu Kim

Produits associés

VCP Polyclonal antibody

10736-1-AP

Fas/CD95 Polyclonal antibody

13098-1-AP

FADD Polyclonal antibody

14906-1-AP

NF-κB p65 Polyclonal antibody

10745-1-AP

CSNK2A1 Polyclonal antibody

10992-1-AP

Caspase 10/p23/17/p12 Polyclonal antibody

14311-1-AP

Caspase 8/p43/p18 Polyclonal antibody

13423-1-AP

FAF2 Polyclonal antibody

16251-1-AP