FAF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FAF2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 16251-1-AP

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

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Validation Data Gallery

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  WB analysis using 16251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16251-1-AP

  WB result of FAF2 antibody (16251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells.

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  IP experiment of mouse brain using 16251-1-AP

  IP result of anti-FAF2 (IP:16251-1-AP, 3ug; Detection:16251-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human breast cancer using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, U2OS cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

16251-1-AP targets FAF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAF2 fusion protein Ag9236
• Full Name: Fas associated factor family member 2
• Calculated Molecular Weight: 445 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014001
• Gene Symbol: FAF2
• Gene ID (NCBI): 23197
• RRID: AB_2262469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

Publications

Species	Application	Title
human	IP,IF	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
		Elife; USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.; Authors - Yanfen Liu
human	WB	Elife; Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling.; Authors - Emma J Fenech
human, bovine	WB	J Biol Chem; A Ubiquitin-like Domain Recruits an Oligomeric Chaperone to a Retrotranslocation Complex in Endoplasmic Reticulum-associated Degradation.; Authors - Xu Yue Y
human	WB	J Biol Chem; Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation.; Authors - Jin-Gu Lee
human	WB	Mol Biol Cell; gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum.; Authors - Ting Zhang

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