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Caspase 8/p43/p18 Polyclonal antibody
Caspase 8/p43/p18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugate
Unconjugated
323
Cat no : 13423-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells |
Positive IP detected in | HeLa cells, Jurkat cells |
Positive IHC detected in | human lymphoma tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 305 publications below |
IHC | See 19 publications below |
IF | See 10 publications below |
IP | See 3 publications below |
ELISA | See 1 publications below |
RIP | See 1 publications below |
Product Information
13423-1-AP targets Caspase 8/p43/p18 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Caspase 8/p43/p18 fusion protein Ag4214 |
Full Name | caspase 8, apoptosis-related cysteine peptidase |
Calculated Molecular Weight | 538 aa, 62 kDa |
Observed Molecular Weight | 53-57 kDa, 32-45 kDa |
GenBank Accession Number | BC028223 |
Gene Symbol | Caspase 8 |
Gene ID (NCBI) | 841 |
RRID | AB_2068463 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Sci Adv HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling. | ||
Exp Mol Med PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer | ||
Nat Commun Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms. | ||
Aging Dis MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis. | ||
EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xiaoping (Verified Customer) (12-15-2021) | Got really good bands in kidney lysates at 1:500 dilution
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FH WEI (Verified Customer) (01-17-2020) | Good antibody for detecting IR injury-induced apoptosis in mouse heart.
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