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Caspase 8/p43/p18 Polyclonal antibody
Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13423-1-AP
Validation Data Gallery
|Positive WB detected in||Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells|
|Positive IP detected in||HeLa cells, Jurkat cells|
|Positive IHC detected in||human lymphoma tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13423-1-AP targets Caspase 8/p43/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken, pig, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 8/p43/p18 fusion protein Ag4214|
|Full Name||caspase 8, apoptosis-related cysteine peptidase|
|Calculated molecular weight||538 aa, 62 kDa|
|Observed molecular weight||53-57 kDa, 32-45 kDa|
|GenBank accession number||BC028223|
|Gene ID (NCBI)||841|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
|Product Specific Protocols|
|WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xiaoping (Verified Customer) (12-15-2021)
Got really good bands in kidney lysates at 1:500 dilution
WEI (Verified Customer) (01-17-2020)
Good antibody for detecting IR injury-induced apoptosis in mouse heart.