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FAS/CD95 Polyclonal antibody

FAS/CD95 Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, CHIP, ELISA

Conjugate

Unconjugated

Cat no : 13098-1-AP

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6



Tested Applications

Positive WB detected inHT-1080 cells, HT-29 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13098-1-AP targets FAS/CD95 in WB, IP, IHC, IF, FC, CoIP, CHIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, sheep, monkey, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAS/CD95 fusion protein Ag3718
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight 35-38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC012479
Gene symbol FAS
Gene ID (NCBI) 355
RRIDAB_2278042
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for FAS/CD95 antibody 13098-1-APDownload protocol
IP protocol for FAS/CD95 antibody 13098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.

Authors - Chang Cui
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
humanWB

Clin Transl Med

c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.

Authors - Lan Zhang
WB

Phytomedicine

Garlic-derived exosomes regulate PFKFB3 expression to relieve liver dysfunction in high-fat diet-fed mice via macrophage-hepatocyte crosstalk

Authors - Jinfan Liu

Diabetes

PMID: 23990362

Authors - Wang Qun Q
WB,IHC

Cancer Lett

Fas signaling in adipocytes promotes low-grade inflammation and lung metastasis of colorectal cancer through interaction with Bmx.

Authors - Fei Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Macarena Lucia (Verified Customer) (11-16-2021)

Some unspecific binding but the target is very easy to identify.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ovarian ca cell lines / melanoma cell lines
FH

Kishor (Verified Customer) (06-23-2019)

This antibody works good at 1:1000 dilution in WB for mouse and human cells.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human and mouse cell lines
FAS/CD95 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in Human and mouse cell lines (Cat no:13098-1-AP)