Caspase 10/p23/17/p12 Polyclonal antibody

Caspase 10/p23/17/p12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14311-1-AP

Synonyms

CASP10, Caspase10, Caspase 10, CASP-10, CASP 10

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Validation Data Gallery

• 

  WB analysis of Jurkat using 14311-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 14311-1-AP (Caspase 10/p23/17/p12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14311-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14311-1-AP (CASP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 14311-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 14311-1-AP (CASP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14311-1-AP

  IP result of anti-Caspase 10/p23/17/p12 (IP:14311-1-AP, 4ug; Detection:14311-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IHC staining of human endometrial cancer using 14311-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14311-1-AP (CASP10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 14311-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14311-1-AP (CASP10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14311-1-AP

  Immunofluorescent analysis of HepG2 cells, using CASP10 antibody 14311-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, Apoptosised HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

14311-1-AP targets Caspase 10/p23/17/p12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 10/p23/17/p12 fusion protein Ag5511
• Full Name: caspase 10, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC042844
• Gene Symbol: Caspase 10
• Gene ID (NCBI): 843
• RRID: AB_2290777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 10 (CASP10) is also called MCH4, is a member of the peptidase C14A family, and contains two death 

effector (DED) domains.

What is the molecular weight of CASP10?

The molecular weight of CASP10 observed on Western blots may vary depending on which of its isoform, 

truncation, or cleavage forms are present. Full-length CASP10 has a propeptide with 219 amino acid and 

6 isoforms produced by alternative splicing.

What is the tissue specificity of CASP10?

CASP10 is detectable in most tissues. However, there is lower expression observed in brain, kidney, colon, 

prostate, and testis tissue.

What is the function of CASP10?

CASP10 plays a role in the cascade of caspases responsible for executing apoptosis. It is recruited to both 

the Fas and TNFR-1 receptors in a FADD dependent manner.  CASP10 is responsible for the cleavage and activation of  

caspases 3, 4, 6, 7, 8, and 9. CASP10 can be cleaved by granzyme B and autocatalytic activity to generate 

two active subunits from its inactive proenzyme form.

What is the role of CASP10 in disease?

Defects in CASP10 lead to autoimmune lymphoproliferative syndrome type 2A (ALPS2A), which is characterized 

by abnormal lymphocyte and dendritic cell homeostasis, as well as immune regulatory defects (PMID:16446975). 

Defects in CASP10 are also a cause of familial non-Hodgkin lymphoma (NHL) and often marked by enlarged lymph 

nodes, weight loss and fever (PMID:12010812), and gastric cancers (GASC) (PMID:11973654).

Publications

Species	Application	Title
human	WB	J Lipid Res; Hyperactivation of SREBP induces Pannexin-1-dependent lytic cell death; Authors - Yanni Xiong
human	WB	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
human	WB	Front Pharmacol; A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.; Authors - Ting Wang
human	WB	Apoptosis; Strong activation of p53 by actinomycin D and nutlin-3a overcomes the resistance of cancer cells to the pro-apoptotic activity of the FAS ligand; Authors - Barbara Łasut-Szyszka

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